MAP4K1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83221

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MAP4K1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LDKLKNPGKGPSIGDIEDEEPELPPAIPRRIRSTHRSSSLGIPDADCCRRHMEFRKLRGMETRPPANTARLQPPRDLRSSSPRKQLSESSDDDYDDVDIPTPAEDTPPPLPPKPKFRSPS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAP4K1 Antibody - BSA Free

Immunohistochemistry-Paraffin: MAP4K1 Antibody [NBP1-83221]

Immunohistochemistry-Paraffin: MAP4K1 Antibody [NBP1-83221]

Immunohistochemistry-Paraffin: MAP4K1 Antibody [NBP1-83221] - Staining of human tonsil shows positivity in germinal center cells.
Immunohistochemistry-Paraffin: MAP4K1 Antibody [NBP1-83221]

Immunohistochemistry-Paraffin: MAP4K1 Antibody [NBP1-83221]

Immunohistochemistry-Paraffin: MAP4K1 Antibody [NBP1-83221] - Staining of human lymph node shows high expression.
Immunohistochemistry-Paraffin: MAP4K1 Antibody [NBP1-83221]

Immunohistochemistry-Paraffin: MAP4K1 Antibody [NBP1-83221]

Immunohistochemistry-Paraffin: MAP4K1 Antibody [NBP1-83221] - Staining in human lymph node and cerebral cortex tissues using anti-MAP4K1 antibody. Corresponding MAP4K1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: MAP4K1 Antibody [NBP1-83221]

Immunohistochemistry-Paraffin: MAP4K1 Antibody [NBP1-83221]

Immunohistochemistry-Paraffin: MAP4K1 Antibody [NBP1-83221] - Staining of human pancreas shows no positivity as expected.
Immunohistochemistry-Paraffin: MAP4K1 Antibody [NBP1-83221]

Immunohistochemistry-Paraffin: MAP4K1 Antibody [NBP1-83221]

Immunohistochemistry-Paraffin: MAP4K1 Antibody [NBP1-83221] - Staining of human colon shows positivity in lymphoid cells.
MAP4K1 Antibody - BSA Free Immunohistochemistry-Paraffin: MAP4K1 Antibody - BSA Free [NBP1-83221]

Immunohistochemistry-Paraffin: MAP4K1 Antibody - BSA Free [NBP1-83221]

Analysis in human lymph node and pancreas tissues using NBP1-83221 antibody. Corresponding MAP4K1 RNA-seq data are presented for the same tissues.

Applications for MAP4K1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAP4K1

The hematopoietic progenitor kinase 1 (HPK1) is a mammalian hematopoiesis-specific Ste20 homologue belonging to the germinal center (GC) kinase family, which contains at least 20 kinases (1). HPK1 is implicated in a variety of signaling systems, including epidermal growth factor, transforming growth factor-beta, erythropoietin, prostaglandin E2, and T cell receptor and B cell receptor stimulation. HPK1 is also involved in Fas ligation-mediated apoptosis, NF-B activation and a highly specific activator of the JNK/SAP signaling pathway (2-5). Phosphorylation of HPK1 on threonine 165, serine 171 and tyrosine 379 is a prerequisite for its enzymatic activation in response to immunoreceptor stimulation (6).

Alternate Names

EC 2.7.11, EC 2.7.11.1, Hematopoietic progenitor kinase, HPK1hematopoietic progenitor kinase 1, MAPK/ERK kinase kinase kinase 1, MEK kinase kinase 1, MEKKK 1, mitogen-activated protein kinase kinase kinase kinase 1

Gene Symbol

MAP4K1

Additional MAP4K1 Products

Product Documents for MAP4K1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MAP4K1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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