MAPKAP Kinase 3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-03381

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MAPKAP Kinase 3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human MAPKAP Kinase 3 (NP_004626.1). MDGETAEEQGGPVPPPVAPGGPGLGGAPGGRREPKKYAVTDDYQLSKQVLGLGVNGKVLECFHRRTGQKCALKLLYDSPKARQEVDHHWQASGGPHIVCILDVYENMHHGKRCLLIIMECMEGGELFSRIQERGDQAFTEREAAEIMRDIGTAIQFLHSHNIAHRDVKPENLLYTSKEKDAVLKLTDFGFAKETTQNALQTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGFP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAPKAP Kinase 3 Antibody - BSA Free

Western Blot: MAPKAP Kinase 3 AntibodyAzide and BSA Free [NBP3-03381]

Western Blot: MAPKAP Kinase 3 AntibodyAzide and BSA Free [NBP3-03381]

Western Blot: MAPKAP Kinase 3 Antibody [NBP3-03381] - Analysis of extracts from normal (control) and MAPKAPK3 knockout (KO) HeLa cells, using MAPKAP Kinase 3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
MAPKAP Kinase 3 Antibody - Azide and BSA Free

Western Blot: MAPKAP Kinase 3 Antibody - Azide and BSA Free [MAPKAP Kinase 3] -

Western Blot: MAPKAP Kinase 3 Antibody - Azide and BSA Free [MAPKAP Kinase 3] - Western blot analysis of various lysates using MAPKAP Kinase 3 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
MAPKAP Kinase 3 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: MAPKAP Kinase 3 Antibody - Azide and BSA Free [MAPKAP Kinase 3] -

Immunocytochemistry/ Immunofluorescence: MAPKAP Kinase 3 Antibody - Azide and BSA Free [MAPKAP Kinase 3] - Immunofluorescence analysis of A549 cells using MAPKAP Kinase 3 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for MAPKAP Kinase 3 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MAPKAP Kinase 3

MAPKAP Kinase 3 encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation.

Alternate Names

3PKEC 2.7.11.1, Chromosome 3p kinase, EC 2.7.11, MAP kinase-activated protein kinase 3, MAPK-activated protein kinase 3, MAPKAP kinase 3, MAPKAP33pK, MAPKAPK-3, mitogen-activated protein kinase-activated protein kinase 3

Gene Symbol

MAPKAPK3

Additional MAPKAP Kinase 3 Products

Product Documents for MAPKAP Kinase 3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MAPKAP Kinase 3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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