MBL Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85518

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MBL Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DGDSSLAASERKALQTEMARIKKWLTFSLGKQVGNKFFLTNGEIMTFEKVKALCVKFQASVATPRNAAENGAIQNLIKEEAFLGITDEKTEGQFVDLTGNRLTYTNWNEGEPNNAGSDEDCVLLLKNGQWNDVPCSTSH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MBL Antibody - BSA Free

Immunohistochemistry-Paraffin: MBL Antibody [NBP1-85518]

Immunohistochemistry-Paraffin: MBL Antibody [NBP1-85518]

Immunohistochemistry-Paraffin: MBL Antibody [NBP1-85518] - Analysis in human liver and prostate tissues using NBP1-85518 antibody. Corresponding MBL RNA-seq data are presented for the same tissues.
Western Blot: MBL Antibody [NBP1-85518]

Western Blot: MBL Antibody [NBP1-85518]

Western Blot: MBL Antibody [NBP1-85518] - Analysis in human liver tissue.
Immunohistochemistry-Paraffin: MBL Antibody [NBP1-85518]

Immunohistochemistry-Paraffin: MBL Antibody [NBP1-85518]

Immunohistochemistry-Paraffin: MBL Antibody [NBP1-85518] - Staining of human cervix, uterine shows no positivity in squamous epithelial cells as expected.
Immunohistochemistry-Paraffin: MBL Antibody [NBP1-85518]

Immunohistochemistry-Paraffin: MBL Antibody [NBP1-85518]

Immunohistochemistry-Paraffin: MBL Antibody [NBP1-85518] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: MBL Antibody [NBP1-85518]

Immunohistochemistry-Paraffin: MBL Antibody [NBP1-85518]

Immunohistochemistry-Paraffin: MBL Antibody [NBP1-85518] - Staining of human cerebral cortex shows no positivity neurons as expected.
Immunohistochemistry-Paraffin: MBL Antibody [NBP1-85518]

Immunohistochemistry-Paraffin: MBL Antibody [NBP1-85518]

Immunohistochemistry-Paraffin: MBL Antibody [NBP1-85518] - Staining of human prostate shows positivity in glandular cells as expected.

Applications for MBL Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MBL

Mannose-binding lectin, MBL, belongs to the C-type family of collectins. It functions as an opsonin which activates the alternative pathway of the complement system on binding to microbial polysaccharides.

Long Name

Mannose Binding Lectin

Alternate Names

COLEC1, Collectin-1, MBL2, MBL2D, MBP1

Gene Symbol

MBL2

Additional MBL Products

Product Documents for MBL Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MBL Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MBL Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MBL Antibody - BSA Free

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  • Q: We are working with the innate immune system of the squid Euprymna scolopes, and we are interested in characterizing the presence and patter of expression of MBL. We know that your company manufactures an antibody against MBL and we were wondering if you think that it may be used to identify the protein in the squid. I am attaching the sequence of the gene that encodes MBL in the squid as a reference for you to compare. Thanks a lot for your help. Squid C-type lectin: QDPRRHSCYYFNNATYLLSDKNLDWKETSKYCRRFHGHLPTPRNKVQVEFLSSNINWSNFNGQRYWIGLVGPSWTWSDGSPLTFANWGHKQPSPKIPGVDTCAYVNVAQQHKWEDMTCTEKKNTGA

    A: I would not recommend using our product NBP1-85518 to detect your squid protein as the homology is very low.

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