MBP Antibody (MBP/4277R) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-08388
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Canine
Applications
Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # MBP/4277R
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant fragment (around aa 150-250) of human MBP (exact sequence is proprietary) (Uniprot: P02686)
Localization
Cell surface, Cytoplasm
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07276).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for MBP Antibody (MBP/4277R) - Azide and BSA Free
Immunohistochemistry-Paraffin: MBP Antibody (MBP/4277R) - Azide and BSA Free [NBP3-08388]
Immunohistochemistry-Paraffin: MBP Antibody (MBP/4277R) [NBP3-08388] - Formalin-fixed, paraffin-embedded human brain stained with MBP Recombinant Rabbit Monoclonal Antibody (MBP/4277R).Immunohistochemistry-Paraffin: MBP Antibody (MBP/4277R) - Azide and BSA Free [NBP3-08388]
Immunohistochemistry-Paraffin: MBP Antibody (MBP/4277R) [NBP3-08388] - Formalin-fixed, paraffin-embedded human brain stained with MBP Recombinant Rabbit Monoclonal Antibody (MBP/4277R).Protein Array: MBP Antibody (MBP/4277R) - Azide and BSA Free [NBP3-08388]
Protein Array: MBP Antibody (MBP/4277R) [NBP3-08388] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MBP Recombinant Rabbit Monoclonal Antibody (MBP/4277R).Western Blot: MBP Antibody (MBP/4277R) - Azide and BSA Free [NBP3-08388] -
Western Blot Analysis of human brain tissue lysate using MBP Antibody (MBP/4277R) - Azide and BSA Free.Applications for MBP Antibody (MBP/4277R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: MBP
Long Name
Myelin Basic Protein
Alternate Names
Hmbpr, mld, shi
Gene Symbol
MBP
Additional MBP Products
Product Documents for MBP Antibody (MBP/4277R) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MBP Antibody (MBP/4277R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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