MCG10 Antibody

Novus Biologicals | Catalog # NB100-68152

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MCG10 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-NGSKKAERQKFSPY corresponding to C-Terminus according to NP_127501.1, NP_127502.1, NP_127503.1.

Epitope

NGSKKAERQKFSPY

Specificity

This antibody is expected to recognise all reported isoforms (NP_065151.1, NP_127501.1, NP_127502.1 and NP_127503.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MCG10 Antibody

Western Blot: MCG10 Antibody [NB100-68152]

Western Blot: MCG10 Antibody [NB100-68152]

Western Blot: MCG10 Antibody [NB100-68152] - Analysis of MCG10 antibody using this antibody at 0.03 ug/ml in HeLa cell lysate. Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: MCG10 Antibody [NB100-68152]

Western Blot: MCG10 Antibody [NB100-68152]

Western Blot: MCG10 Antibody [NB100-68152] - HEK293 overexpressing PCBP4 and probed with (mock transfection in first lane).

Applications for MCG10 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 40 kDa band observed in HeLa lysates (calculated MW of 41.4 kDa band according to NP_127502.1 and NP_127503.1). In transfected HEK293 transiently expressing PCBP4 a band of approx. 45 kDa is observed. This band is not observed in the non-transfected HEK293.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MCG10

This gene encodes a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. This gene is induced by the p53 tumor suppressor, and the encoded protein can suppress cell proliferation by inducing apoptosis and cell cycle arrest in G(2)-M. This gene's protein is found in the cytoplasm, yet it lacks the nuclear localization signals found in other subfamily members. Multiple alternatively spliced transcript variants have been described, but the full-length nature for only some has been determined.

Alternate Names

Alpha-CP4, alphaCP-4 protein, CBP, LIP4, LYST-interacting protein, MCG10, poly(rC) binding protein 4, poly(rC)-binding protein 4, RNA binding protein MCG10

Entrez Gene IDs

57060 (Human); 59092 (Mouse); 363133 (Rat)

Gene Symbol

PCBP4

UniProt

NP_065151.1 (Human)

Additional MCG10 Products

Product Documents for MCG10 Antibody

Certificate of Analysis

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Product Specific Notices for MCG10 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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