mCherry Antibody (1C51) - BSA Free

A:

At this time we do not have any date on the use of NBP1-96752 in Immunohistochemistry. If you would be interested in testing this antibody, I would invite you to take a look at our Innovator's Reward Program.

A: mCherry and GFP share just 29% sequence similarity, so this antibody is not predicted to cross-react to GFP and has never shown any ability to detect GFP in testing.

A:

mCherry differs from other GFP-derived proteins by maturing extremely rapidly, and is highly photostable and resists photobleaching. (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910338/).

In short this protein does not naturally occur in the higher mammals, and this is relevant to answer your concern. Since not present in the higher mammals, the only way for our lab to easily assess whether NBP1-96752 was specific to mCharry protein was using transiently transfection of its gene (in pFin-EF1-mCherry vector) into the host of interests, in this case HEK293 cells. Therefore, the crude extract of HEk293 cells transfected with the gene (lane + on the posted WB image) could reveal the mCherry species by the antibody, but an equal amount of protein extract from untransfected HEK293 cells (lane -) could not.

In these experiments, it was not the endogenous protein was detected. The transiently expressed protein may be associated with many detection artifacts, namely, degradation easier, pre-maturation termination of transcription/translation, aggression of protein due to over expression of the proteins in situ, and many others. All of these might be contributing to the detection of the mCherry bands, as those see on the image.
Since this antibody have been cited in many publications (9), 8 of which were using the antibody in WB, we are confident that the quality of NBP1-96752 is good. In fact we 100% guarantee it to produce the positive results in WB. Or we will refund or free replacement of any primary antibody with the similar price.

A: I am sorry, but product NBP1-97373 has been discontinued. However, I have now received confirmation from the lab that mCherry Antibody (1C51) NBP1-96752 does indeed bind to dTomato, and so we would recommend this antibody for you.

A: We have not tested our mCherry antibody with catalogue number NBP1-96752 against RFP, however since the two proteins share a high degree of sequence homology the antibody is likely to recognise RFP. As we have not performed this testing in house however, we cannot guarantee that NBP1-96752 will or will not cross-react with RFP.

A: NBP1-45840, NBP1-97373 and NBP1-97371 will all recognize DsRed. I have no information about its cross-reactivity of DsRed with NBP1-96752.

A:

At this time we do not have any date on the use of NBP1-96752 in Immunohistochemistry. If you would be interested in testing this antibody, I would invite you to take a look at our Innovator's Reward Program.

A: mCherry and GFP share just 29% sequence similarity, so this antibody is not predicted to cross-react to GFP and has never shown any ability to detect GFP in testing.

A:

mCherry differs from other GFP-derived proteins by maturing extremely rapidly, and is highly photostable and resists photobleaching. (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910338/).

In short this protein does not naturally occur in the higher mammals, and this is relevant to answer your concern. Since not present in the higher mammals, the only way for our lab to easily assess whether NBP1-96752 was specific to mCharry protein was using transiently transfection of its gene (in pFin-EF1-mCherry vector) into the host of interests, in this case HEK293 cells. Therefore, the crude extract of HEk293 cells transfected with the gene (lane + on the posted WB image) could reveal the mCherry species by the antibody, but an equal amount of protein extract from untransfected HEK293 cells (lane -) could not.

In these experiments, it was not the endogenous protein was detected. The transiently expressed protein may be associated with many detection artifacts, namely, degradation easier, pre-maturation termination of transcription/translation, aggression of protein due to over expression of the proteins in situ, and many others. All of these might be contributing to the detection of the mCherry bands, as those see on the image.
Since this antibody have been cited in many publications (9), 8 of which were using the antibody in WB, we are confident that the quality of NBP1-96752 is good. In fact we 100% guarantee it to produce the positive results in WB. Or we will refund or free replacement of any primary antibody with the similar price.

A: I am sorry, but product NBP1-97373 has been discontinued. However, I have now received confirmation from the lab that mCherry Antibody (1C51) NBP1-96752 does indeed bind to dTomato, and so we would recommend this antibody for you.

A: We have not tested our mCherry antibody with catalogue number NBP1-96752 against RFP, however since the two proteins share a high degree of sequence homology the antibody is likely to recognise RFP. As we have not performed this testing in house however, we cannot guarantee that NBP1-96752 will or will not cross-react with RFP.

A: NBP1-45840, NBP1-97373 and NBP1-97371 will all recognize DsRed. I have no information about its cross-reactivity of DsRed with NBP1-96752.

A:

At this time we do not have any date on the use of NBP1-96752 in Immunohistochemistry. If you would be interested in testing this antibody, I would invite you to take a look at our Innovator's Reward Program.

A: mCherry and GFP share just 29% sequence similarity, so this antibody is not predicted to cross-react to GFP and has never shown any ability to detect GFP in testing.

A:

mCherry differs from other GFP-derived proteins by maturing extremely rapidly, and is highly photostable and resists photobleaching. (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910338/).

In short this protein does not naturally occur in the higher mammals, and this is relevant to answer your concern. Since not present in the higher mammals, the only way for our lab to easily assess whether NBP1-96752 was specific to mCharry protein was using transiently transfection of its gene (in pFin-EF1-mCherry vector) into the host of interests, in this case HEK293 cells. Therefore, the crude extract of HEk293 cells transfected with the gene (lane + on the posted WB image) could reveal the mCherry species by the antibody, but an equal amount of protein extract from untransfected HEK293 cells (lane -) could not.

In these experiments, it was not the endogenous protein was detected. The transiently expressed protein may be associated with many detection artifacts, namely, degradation easier, pre-maturation termination of transcription/translation, aggression of protein due to over expression of the proteins in situ, and many others. All of these might be contributing to the detection of the mCherry bands, as those see on the image.
Since this antibody have been cited in many publications (9), 8 of which were using the antibody in WB, we are confident that the quality of NBP1-96752 is good. In fact we 100% guarantee it to produce the positive results in WB. Or we will refund or free replacement of any primary antibody with the similar price.

A: I am sorry, but product NBP1-97373 has been discontinued. However, I have now received confirmation from the lab that mCherry Antibody (1C51) NBP1-96752 does indeed bind to dTomato, and so we would recommend this antibody for you.

A: We have not tested our mCherry antibody with catalogue number NBP1-96752 against RFP, however since the two proteins share a high degree of sequence homology the antibody is likely to recognise RFP. As we have not performed this testing in house however, we cannot guarantee that NBP1-96752 will or will not cross-react with RFP.

A: NBP1-45840, NBP1-97373 and NBP1-97371 will all recognize DsRed. I have no information about its cross-reactivity of DsRed with NBP1-96752.

A:

At this time we do not have any date on the use of NBP1-96752 in Immunohistochemistry. If you would be interested in testing this antibody, I would invite you to take a look at our Innovator's Reward Program.

A: mCherry and GFP share just 29% sequence similarity, so this antibody is not predicted to cross-react to GFP and has never shown any ability to detect GFP in testing.

A:

mCherry differs from other GFP-derived proteins by maturing extremely rapidly, and is highly photostable and resists photobleaching. (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910338/).

In short this protein does not naturally occur in the higher mammals, and this is relevant to answer your concern. Since not present in the higher mammals, the only way for our lab to easily assess whether NBP1-96752 was specific to mCharry protein was using transiently transfection of its gene (in pFin-EF1-mCherry vector) into the host of interests, in this case HEK293 cells. Therefore, the crude extract of HEk293 cells transfected with the gene (lane + on the posted WB image) could reveal the mCherry species by the antibody, but an equal amount of protein extract from untransfected HEK293 cells (lane -) could not.

In these experiments, it was not the endogenous protein was detected. The transiently expressed protein may be associated with many detection artifacts, namely, degradation easier, pre-maturation termination of transcription/translation, aggression of protein due to over expression of the proteins in situ, and many others. All of these might be contributing to the detection of the mCherry bands, as those see on the image.
Since this antibody have been cited in many publications (9), 8 of which were using the antibody in WB, we are confident that the quality of NBP1-96752 is good. In fact we 100% guarantee it to produce the positive results in WB. Or we will refund or free replacement of any primary antibody with the similar price.

A: I am sorry, but product NBP1-97373 has been discontinued. However, I have now received confirmation from the lab that mCherry Antibody (1C51) NBP1-96752 does indeed bind to dTomato, and so we would recommend this antibody for you.

A: We have not tested our mCherry antibody with catalogue number NBP1-96752 against RFP, however since the two proteins share a high degree of sequence homology the antibody is likely to recognise RFP. As we have not performed this testing in house however, we cannot guarantee that NBP1-96752 will or will not cross-react with RFP.

A: NBP1-45840, NBP1-97373 and NBP1-97371 will all recognize DsRed. I have no information about its cross-reactivity of DsRed with NBP1-96752.

A:

At this time we do not have any date on the use of NBP1-96752 in Immunohistochemistry. If you would be interested in testing this antibody, I would invite you to take a look at our Innovator's Reward Program.

A: mCherry and GFP share just 29% sequence similarity, so this antibody is not predicted to cross-react to GFP and has never shown any ability to detect GFP in testing.

A:

mCherry differs from other GFP-derived proteins by maturing extremely rapidly, and is highly photostable and resists photobleaching. (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910338/).

In short this protein does not naturally occur in the higher mammals, and this is relevant to answer your concern. Since not present in the higher mammals, the only way for our lab to easily assess whether NBP1-96752 was specific to mCharry protein was using transiently transfection of its gene (in pFin-EF1-mCherry vector) into the host of interests, in this case HEK293 cells. Therefore, the crude extract of HEk293 cells transfected with the gene (lane + on the posted WB image) could reveal the mCherry species by the antibody, but an equal amount of protein extract from untransfected HEK293 cells (lane -) could not.

In these experiments, it was not the endogenous protein was detected. The transiently expressed protein may be associated with many detection artifacts, namely, degradation easier, pre-maturation termination of transcription/translation, aggression of protein due to over expression of the proteins in situ, and many others. All of these might be contributing to the detection of the mCherry bands, as those see on the image.
Since this antibody have been cited in many publications (9), 8 of which were using the antibody in WB, we are confident that the quality of NBP1-96752 is good. In fact we 100% guarantee it to produce the positive results in WB. Or we will refund or free replacement of any primary antibody with the similar price.

A: I am sorry, but product NBP1-97373 has been discontinued. However, I have now received confirmation from the lab that mCherry Antibody (1C51) NBP1-96752 does indeed bind to dTomato, and so we would recommend this antibody for you.

A: We have not tested our mCherry antibody with catalogue number NBP1-96752 against RFP, however since the two proteins share a high degree of sequence homology the antibody is likely to recognise RFP. As we have not performed this testing in house however, we cannot guarantee that NBP1-96752 will or will not cross-react with RFP.

A: NBP1-45840, NBP1-97373 and NBP1-97371 will all recognize DsRed. I have no information about its cross-reactivity of DsRed with NBP1-96752.

A:

At this time we do not have any date on the use of NBP1-96752 in Immunohistochemistry. If you would be interested in testing this antibody, I would invite you to take a look at our Innovator's Reward Program.

A: mCherry and GFP share just 29% sequence similarity, so this antibody is not predicted to cross-react to GFP and has never shown any ability to detect GFP in testing.

A:

mCherry differs from other GFP-derived proteins by maturing extremely rapidly, and is highly photostable and resists photobleaching. (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910338/).

In short this protein does not naturally occur in the higher mammals, and this is relevant to answer your concern. Since not present in the higher mammals, the only way for our lab to easily assess whether NBP1-96752 was specific to mCharry protein was using transiently transfection of its gene (in pFin-EF1-mCherry vector) into the host of interests, in this case HEK293 cells. Therefore, the crude extract of HEk293 cells transfected with the gene (lane + on the posted WB image) could reveal the mCherry species by the antibody, but an equal amount of protein extract from untransfected HEK293 cells (lane -) could not.

In these experiments, it was not the endogenous protein was detected. The transiently expressed protein may be associated with many detection artifacts, namely, degradation easier, pre-maturation termination of transcription/translation, aggression of protein due to over expression of the proteins in situ, and many others. All of these might be contributing to the detection of the mCherry bands, as those see on the image.
Since this antibody have been cited in many publications (9), 8 of which were using the antibody in WB, we are confident that the quality of NBP1-96752 is good. In fact we 100% guarantee it to produce the positive results in WB. Or we will refund or free replacement of any primary antibody with the similar price.

A: I am sorry, but product NBP1-97373 has been discontinued. However, I have now received confirmation from the lab that mCherry Antibody (1C51) NBP1-96752 does indeed bind to dTomato, and so we would recommend this antibody for you.

A: We have not tested our mCherry antibody with catalogue number NBP1-96752 against RFP, however since the two proteins share a high degree of sequence homology the antibody is likely to recognise RFP. As we have not performed this testing in house however, we cannot guarantee that NBP1-96752 will or will not cross-react with RFP.

A: NBP1-45840, NBP1-97373 and NBP1-97371 will all recognize DsRed. I have no information about its cross-reactivity of DsRed with NBP1-96752.