MDA5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89685

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all IFIH1/MDA5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TIRMIDAYTHLETFYNEEKDKKFAVIEDDSDEGGDDEYCDGDEDEDDLKKPLKLDETDRFLMTLFFENNKMLKRLAENPEYENEKLTKLRNTIMEQYTRTEESARGIIFTKTRQSAYALSQWITENEKFAEVGVKAHHLIGAGHSSEFKP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MDA5 Antibody - BSA Free

MDA5 Antibody - BSA Free Immunohistochemistry-Paraffin: MDA5 Antibody - BSA Free [NBP1-89685]

Immunohistochemistry-Paraffin: MDA5 Antibody - BSA Free [NBP1-89685]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
MDA5 Antibody - BSA Free Immunohistochemistry-Paraffin: MDA5 Antibody - BSA Free [NBP1-89685]

Immunohistochemistry-Paraffin: MDA5 Antibody - BSA Free [NBP1-89685]

Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.
MDA5 Antibody - BSA Free Immunohistochemistry-Paraffin: MDA5 Antibody - BSA Free [NBP1-89685]

Immunohistochemistry-Paraffin: MDA5 Antibody - BSA Free [NBP1-89685]

Staining of human pancreas shows moderate granular cytoplasmic positivity in exocrine glandular cells.
MDA5 Antibody - BSA Free Immunohistochemistry-Paraffin: MDA5 Antibody - BSA Free [NBP1-89685]

Immunohistochemistry-Paraffin: MDA5 Antibody - BSA Free [NBP1-89685]

Staining of human placenta shows moderate granular cytoplasmic positivity in trophoblastic cells.
MDA5 Antibody - BSA Free Western Blot: MDA5 Antibody - BSA Free [NBP1-89685]

Western Blot: MDA5 Antibody - BSA Free [NBP1-89685]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line SK-MEL-30

Applications for MDA5 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IFIH1/MDA5

Gene ID: 64135 DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation.

Long Name

Melanoma Differentiation-Associated Protein 5

Alternate Names

AGS7, Helicard, Hlcd, IDDM19, IFIH1, IMD95, MDA-5, RH116, SGMRT1

Gene Symbol

IFIH1

Additional IFIH1/MDA5 Products

Product Documents for MDA5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MDA5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Infectious Diseases

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Protocols

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FAQs

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