MDH1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77780

Novus Biologicals
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Key Product Details

Species Reactivity

Porcine

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Format

BSA Free
View all MDH1 Primary Antibodies »

Product Specifications

Immunogen

MDH1 [Pig Heart] (Uniprot: P11708)

Reactivity Notes

Cross reactivity against MDH1 from other tissues and species may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for MDH1 Antibody - BSA Free

Western Blot: MDH1 Antibody [NBP1-77780]

Western Blot: MDH1 Antibody [NBP1-77780]

Western Blot: MDH1 Antibody [NBP1-77780] - Malate Dehydrogenase Antibody
MDH1 Antibody

MDH1 Antibody

Western Blot of Sheep anti-Malate Dehydrogenase antibody. Lane 1: Malate Dehydrogenase. Lane 2: None. Load: 50 ng per lane. Primary antibody: Malate Dehydrogenase antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase sheep secondary antibody at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 36 kDa, 36 kDa for Malate Dehydrogenase. Other band(s): None

Applications for MDH1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:5000-1:20000

Immunoprecipitation

1:100

Western Blot

1:500-1:5000
Application Notes
This product has been tested by western blot and is suitable to be assayed against 1.0 ug of Malate Dehydrogenase [Pig Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MDH1

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. (provided by RefSeq)

Long Name

malate dehydrogenase 1

Alternate Names

DEE88, EIEE88, HEL-S-32, KAR, MDH-s, MDHA, MGC:1375, MOR2

Gene Symbol

MDH1

UniProt

P11708 (Porcine)

Additional MDH1 Products

Product Documents for MDH1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MDH1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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