MDH1 Antibody [HRP]

Novus Biologicals | Catalog # NB100-2019

Novus Biologicals
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Key Product Details

Species Reactivity

Porcine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

HRP

Antibody Source

Polyclonal Sheep IgG
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Product Specifications

Immunogen

MDH1 [Pig Heart] (Uniprot: P11708)

Reactivity Notes

Cross reactivity against MDH1 from other tissues and species may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Malate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart]

Scientific Data Images for MDH1 Antibody [HRP]

Western Blot: MDH1 Antibody [HRP] [NB100-2019] - Western Blot of Sheep MDH1 antibody [HRP]. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: MDH1 [0.05ug]. Primary Antibody: Anti-MDH1 HRP at 1:1000 for 1 hr at RT. Secondary Antibody: none. Blocking Buffer for 30mins at RT. Predicted MW: 30-35kDa. Exposure: 1sec.

Applications for MDH1 Antibody [HRP]

Application
Recommended Usage

ELISA

1:10000-1:50000

Immunohistochemistry

1:500-1:2500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:100

Western Blot

1:1000-1:5000
Application Notes
This product has been tested by western blot and is useful in ELISA and immunohistochemistry. Optimal titers should be obtained by researchers.

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Gentamicin Sulfate

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MDH1

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. (provided by RefSeq)

Long Name

malate dehydrogenase 1

Alternate Names

DEE88, EIEE88, HEL-S-32, KAR, MDH-s, MDHA, MGC:1375, MOR2

Gene Symbol

MDH1

Additional MDH1 Products

Product Documents for MDH1 Antibody [HRP]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MDH1 Antibody [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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