MDR1/ABCB1 Antibody (OTI1A7)
Novus Biologicals | Catalog # NBP2-46466
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Goat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI1A7
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Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 995-1280 of human ABCB1 (NP_000918) produced in SF9 cell.
Reactivity Notes
Goat reactivity reported in scientific literature (Agarwal T et al). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for MDR1/ABCB1 Antibody (OTI1A7)
![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0015.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0001.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0002.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0003.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0004.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0005.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0006.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0007.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human lung tissue within the normal limits using ABCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0008.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0009.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0010.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0011.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0012.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0013.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0014.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0016.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)Applications for MDR1/ABCB1 Antibody (OTI1A7)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Reported in scientific literature (PMID: 30179804)
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MDR1/ABCB1
Long Name
ATP-binding Cassette, Sub-family B (MDR/TAP), Member 1B
Alternate Names
ABC20, ABCB1, ABCB1B, CD243, CLCS, GP170, IBD13, PGY1
Gene Symbol
ABCB1
Additional MDR1/ABCB1 Products
Product Documents for MDR1/ABCB1 Antibody (OTI1A7)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MDR1/ABCB1 Antibody (OTI1A7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for MDR1/ABCB1 Antibody (OTI1A7)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Blood-Brain Barrier Pathway: Anatomy
