MDR1/ABCB1 Antibody (OTI1A7)
Novus Biologicals | Catalog # NBP2-46466
![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0015.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Goat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI1A7
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Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 995-1280 of human ABCB1 (NP_000918) produced in SF9 cell.
Reactivity Notes
Goat reactivity reported in scientific literature (Agarwal T et al). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for MDR1/ABCB1 Antibody (OTI1A7)
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0001.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0002.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0003.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0004.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0005.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0006.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0007.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human lung tissue within the normal limits using ABCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0008.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0009.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0010.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0011.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0012.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0013.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0014.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]](https://resources.rndsystems.com/images/products/MDR1-ABCB1-Antibody-1A7-Immunohistochemistry-NBP2-46466-img0016.jpg "Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]")
Immunohistochemistry: MDR1/ABCB1 Antibody (OTI1A7) [NBP2-46466]
Immunohistochemistry: MDR1/ABCB1 Antibody (1A7) [NBP2-46466] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)Applications for MDR1/ABCB1 Antibody (OTI1A7)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Reported in scientific literature (PMID: 30179804)
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MDR1/ABCB1
Long Name
ATP-binding Cassette, Sub-family B (MDR/TAP), Member 1B
Alternate Names
ABC20, ABCB1, ABCB1B, CD243, CLCS, GP170, IBD13, PGY1
Gene Symbol
ABCB1
Additional MDR1/ABCB1 Products
Product Documents for MDR1/ABCB1 Antibody (OTI1A7)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MDR1/ABCB1 Antibody (OTI1A7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for MDR1/ABCB1 Antibody (OTI1A7)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
