MDR1/ABCB1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-17251

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Feline (92%), Rabbit (93%), Rhesus Macaque (93%), Sheep (93%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MDR1/ABCB1 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MDR1/ABCB1. The exact sequence is proprietary.

Reactivity Notes

Cat (92%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

141 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MDR1/ABCB1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MDR1/ABCB1 Antibody [NBP2-17251]

Immunocytochemistry/ Immunofluorescence: MDR1/ABCB1 Antibody [NBP2-17251]

Immunocytochemistry/Immunofluorescence: MDR1/ABCB1 Antibody [NBP2-17251] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: P Glycoprotein protein stained by P Glycoprotein antibody diluted at 1:500. Blue: Hoechst 33342 staining.
MDR1/ABCB1 Antibody

Western Blot: MDR1/ABCB1 Antibody [NBP2-17251] -

Western Blot: MDR1/ABCB1 Antibody [NBP2-17251] - Unboiled HepG2 whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MDR1/ABCB1 antibody (NBP2-17251) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Applications for MDR1/ABCB1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MDR1/ABCB1

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. [provided by RefSeq]

Long Name

ATP-binding Cassette, Sub-family B (MDR/TAP), Member 1B

Alternate Names

ABC20, ABCB1, ABCB1B, CD243, CLCS, GP170, IBD13, PGY1

Gene Symbol

ABCB1

UniProt

P08183 (Human)

Additional MDR1/ABCB1 Products

Product Documents for MDR1/ABCB1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MDR1/ABCB1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for MDR1/ABCB1 Antibody - BSA Free

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Protocols

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FAQs

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