MED13 Antibody

Novus Biologicals | Catalog # NB100-60642

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Primate

Cited:

Human, Primate

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all MED13 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 750 and 800 of human thyroid hormone receptor associated protein 1 (thyroid hormone receptor-associated protein, 240 kDa) using the numbering given in entry NP_005112.2 (GeneID 996

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 24147125).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MED13 Antibody

Western Blot: MED13 Antibody [NB100-60642]

Western Blot: MED13 Antibody [NB100-60642]

Western Blot: MED13 Antibody [NB100-60642] - Whole cell lysate from HeLa and 293T cells. THRAP1/TRAP240 was also immunoprecipitated by rabbit anti-THRAP1/TRAP240 antibody NB100-60641.
MED13 Antibody

Western Blot: MED13 Antibody [NB100-60642] -

TAP-n208 associates with both TFIID and Mediator.(A) Western blot analysis of nuclear extracts (NE) or affinity purified (PD) samples collected at 6 hpi using either wtICP4 (KOS), TAP-wtICP4 (TAP), the C-terminal truncation mutant (n208) or a TAP-tagged C-terminal truncation mutant (TAP-n208) expressing viruses. Antibodies directed against ICP4, TAF1 and TBP of TFIID, and Med1 of Mediator were used. (B) Quantification of the western blots in (A). Images were quantified using the LiCOR Odyssey infrared imager and normalized to the amount of ICP4 present within the sample. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24147125), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
MED13 Antibody

Western Blot: MED13 Antibody [NB100-60642] -

TFIID and Mediator display different temporal associations with ICP4.(A) Western blot analysis of nuclear extracts (NE) or affinity purified (PD) samples collected at either 3 hpi or 6 hpi using either wtICP4 (KOS) or TAP-wtICP4 (TAP) expressing viruses. Antibodies directed against ICP4, TAF1 and TBP of TFIID, and Med1 of Mediator were used. (B) Quantification of the western blots in (A). Images were quantified using the LiCOR Odyssey infrared imager and normalized to the amount of ICP4 present within the sample. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24147125), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for MED13 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MED13

This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator.

Alternate Names

Activator-recruited cofactor 250 kDa component, KIAA0593HSPC221, mediator complex subunit 13ARC250, mediator of RNA polymerase II transcription, subunit 13 homolog, THRAP1mediator of RNA polymerase II transcription subunit 13, thyroid hormone receptor associated protein 1, Thyroid hormone receptor-associated protein 1, Thyroid hormone receptor-associated protein complex 240 kDa component, thyroid hormone receptor-associated protein complex component TRAP240, thyroid hormone receptor-associated protein, 240 kDa subunit, Trap240, TRAP240DRIP250, Vitamin D3 receptor-interacting protein complex component DRIP250

Entrez Gene IDs

9969 (Human)

Gene Symbol

MED13

UniProt

NP_005112.2 (Human)

Additional MED13 Products

Product Documents for MED13 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MED13 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for MED13 Antibody

There are currently no reviews for this product. Be the first to review MED13 Antibody and earn rewards!

Have you used MED13 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies