MED25 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-83188

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Format

BSA Free
View all MED25 Primary Antibodies »

Product Specifications

Immunogen

The immunogen is a synthetic peptide directed towards the N-terminal region of human MED25. Peptide sequence: EGLRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYV The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for MED25 Antibody - BSA Free

Western Blot: MED25 Antibody [NBP2-83188]

Western Blot: MED25 Antibody [NBP2-83188]

Western Blot: MED25 Antibody [NBP2-83188] - WB Suggested Anti-MED25 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human heart
Chromatin Immunoprecipitation: MED25 Antibody [NBP2-83188] - Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

Applications for MED25 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MED25

Alternate Names

ACID1, Activator Interaction Domain-Containing Protein 1, Activator-Recruited Cofactor 92 KDa Component, ARC/Mediator Transcriptional Coactivator Subunit, ARC92, BVSYS, CMT2B2, Mediator Complex Subunit 25, Mediator Of RNA Polymerase II Transcription Subunit 25, Mediator Of RNA Polymerase II Transcription, Subunit 25 Homolog, Mediator Of RNA Polymerase II Transcription, Subunit 25 Homolog (S. Cerevisiae), P78, TCBAP0758

Gene Symbol

MED25

Additional MED25 Products

Product Documents for MED25 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MED25 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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