MEKK1 Antibody (2F6)

Novus Biologicals | Catalog # NBP2-44405

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F6
View all MEKK1 Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant MEKK1 (aa1077-1176) (SKNSMTLDLNSSSKCDDSFGCSSNSSNAVIPSDETVFTP-VEEKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEKAENDDTYKDDVNHNQK) (Uniprot: Q13233)

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47810)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for MEKK1 Antibody (2F6)

Immunohistochemistry-Paraffin: MEKK1 Antibody (2F6) [NBP2-44405]

Immunohistochemistry-Paraffin: MEKK1 Antibody (2F6) [NBP2-44405]

Immunohistochemistry-Paraffin: MEKK1 Antibody (2F6) [NBP2-44405] - Human Uterine Carcinoma stained with MAP3K1 Monoclonal Antibody (2F6).
Immunohistochemistry-Paraffin: MEKK1 Antibody (2F6) [NBP2-44405]

Immunohistochemistry-Paraffin: MEKK1 Antibody (2F6) [NBP2-44405]

Immunohistochemistry-Paraffin: MEKK1 Antibody (2F6) [NBP2-44405] - Human Cervical Carcinoma stained with MAP3K1 Monoclonal Antibody (2F6).
Immunohistochemistry-Paraffin: MEKK1 Antibody (2F6) [NBP2-44405]

Immunohistochemistry-Paraffin: MEKK1 Antibody (2F6) [NBP2-44405]

Immunohistochemistry-Paraffin: MEKK1 Antibody (2F6) [NBP2-44405] - Human Thyroid Carcinoma stained with MAP3K1 Monoclonal Antibody (2F6).

Applications for MEKK1 Antibody (2F6)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-44405 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MEKK1

MEKKs (Mitogen activated protein kinase kinase kinases) are serine-threonine kinases that act as the first tier of cellular MAP kinase pathways by activation of MAP/ERK kinases, or MEKs. Many enzymes with MEKK activity have been identified, including MEKK1-4, Raf, MLK3, TAK, and DLK. MEKKs generally display little similarity outside of their catalytic kinase domains. MEKK1-4 are nearly 50% identical within their catalytic domains, and are known to regulate Erk, Jnk, and p38 MAP kinase pathways. MEKK2 and MEKK3 bind MEK5 via conserved PB1 domains, leading to downstream activation of Erk5.

Alternate Names

Map3k1, MAPK/ERK kinase kinase 1, MAPKKK1MAP/ERK kinase kinase 1, MEK kinase 1, MEKK1EC 2.7.11.25, MEKKMEKK 1, mitogen-activated protein kinase kinase kinase 1

Gene Symbol

MAP3K1

UniProt

Q13233 (Human)

Additional MEKK1 Products

Product Documents for MEKK1 Antibody (2F6)

Certificate of Analysis

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Product Specific Notices for MEKK1 Antibody (2F6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MEKK1 Antibody (2F6)

Customer Reviews for MEKK1 Antibody (2F6) (1)

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  • Review of MEKK1 antibody for WB
    Name: Anonymous
    Application: Western Blot
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 12/04/2024
    Standard curve of MEKK1 antibody in valvular interstitial cells. The intensity of the band correlates with the amount of total protein of each band.
    The MEKK1 (NBP2-44405) antibody was used on valvular interstitial cells (fibroblasts) with 5, 10 and 15 µg of total proteins at a concentration of 1:1000.
    MEKK1 Antibody (2F6) NBP2-44405

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