Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [Janelia Fluor® 549]

Novus Biologicals | Catalog # NBP2-34681JF549

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Janelia Fluor 549

Antibody Source

Monoclonal Mouse IgG2b Lambda/IgG2a Kappa/IgG1 Kappa Clone # M2-7C10 + M2-9E3 + T311 + HMB45
View all Melanoma Marker (MART-1 + Tyrosinase + gp100) Primary Antibodies »

Product Specifications

Immunogen

Recombinant Melanoma Marker (MART-1 + Tyrosinase + gp100) protein (M2-7C10; M2-9E3); Recombinant tyrosinase protein (T311); Extract of pigmented melanoma metastases from lymph nodes (HMB45)

Localization

Cytoplasmic

Marker

Melanoma Marker

Specificity

This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Lambda/IgG2a Kappa/IgG1 Kappa

Scientific Data Images

Immunocytochemistry/Immunofluorescence: Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [Janelia Fluor® 549] [NBP2-34681JF549] - Scan of human melanoma sample. Stained for Mart-1+Tyrosinase+gp100 (green) counter stained with DAPI (blue). Image from verified customer review.

Applications

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. Successful se in ICC/IF reported by customer review.

Reviewed Applications

Read 1 review rated 5 using NBP2-34681JF549 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Melanoma Marker (MART-1 + Tyrosinase + gp100)

Alternate Names

MART1, MART-1, melan-A, MLANA

Gene Symbol

MLANA

Additional Melanoma Marker (MART-1 + Tyrosinase + gp100) Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews (1)

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  • Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [Janelia Fluor® 549]
    Name: Jan Martinek
    Application: Immunofluorescence
    Sample Tested: human melanoma
    Species: Human
    Verified Customer | Posted 06/03/2019
    Scan of human melanoma sample. Stained for Mart-1+Tyrosinase+gp100 (green) counter stained with DAPI (blue).
    Acetone fixation
    Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody (M2-7C10 + M2-9E3 + T311 + HMB45) [Janelia Fluor® 549] NBP2-34681JF549

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Protocols

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FAQs

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