MELK Antibody (PSH03-55)

Immunohistochemistry: MELK Antibody (PSH03-55) [NBP3-32587]

Immunohistochemistry: MELK Antibody (PSH03-55) [NBP3-32587] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-MELK antibody (NBP3-32587) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32587) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: MELK Antibody (PSH03-55) [NBP3-32587]

Western blot analysis on different lysates with NBP3-32587 at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: HCT 116 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C2C12 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 75 kDa Observed band size: 72 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32587 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.