MEM-Alpha, 25 mM HEPES, nucleosides, GlutaminePlus

Eagle’s Minimum Essential Medium (MEM), a modification of Eagle’s BME, is one of the most commonly used cell culture media. MEM is suitable for a broad spectrum of mammalian cells in culture. In comparison with BME, it contains higher concentrations of amino acids and other essential nutrients. MEM is available with Earle’s salts for use in a CO<sub>2 incubator, with Hanks’ salts for use without CO2, with or without non-essential amino acids, or as Alpha modification with or without nucleosides.

Each lot of MEM Alpha is prepared from powdered base medium, tissue culture-grade water, and is sterile filtered using a 0.2 micron filter. Representative samples of each lot of MEM Alpha are tested to confirm the absence of bacterial or fungal contamination using methods adapted from the current U.S. Pharmacopeia. MEM Alpha is manufactured in our ISO 9001:2015 certified facility.

For the specific media formulation, please refer to the Media Formulation section of the datasheet.</sub>