If culturing in the absence of a feeder cell layer is desired, human embryonic stem (hES) cells can be maintained using Mouse Conditioned Media (Catalog # AR005). The protocol below has been used with the BG01V line of hES cells. Please note that other hES cell lines may require modifications of this protocol. Optimal culture conditions must be determined by the investigator for each hES line.
Please read the protocol in its entirety before starting.
Note: When handling biohazard materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.
Note: Sterile technique is required when handling the reagents.
BG01V cells are licensed from ViaCyte, Inc.
Cultrex is a registered trademark of Trevigen, Inc.