Methyl-Lysine Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NB600-824

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Non-species specific

Cited:

Human, Porcine

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Methyl-Lysine Primary Antibodies »

Product Specifications

Immunogen

Methylated KLH Conjugated

Specificity

Detects proteins containing methylated lysine residues.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Methyl-Lysine Antibody - Azide and BSA Free

Western Blot: Methyl-Lysine Antibody [NB600-824]

Western Blot: Methyl-Lysine Antibody [NB600-824]

Western Blot: Methyl-Lysine Antibody [NB600-824] - Western blot analysis of Bovine serum albumin showing detection of Methyl-Lysine protein using Rabbit Anti-Methyl-Lysine Polyclonal Antibody (NB600-824). Primary Antibody: Rabbit Anti-Methyl-Lysine Polyclonal Antibody (NB600-824) at 1:1000. Methyl-Lysine in BSA (Left) and Methylated BSA (Right).
Immunocytochemistry/ Immunofluorescence: Methyl-Lysine Antibody [NB600-824]

Immunocytochemistry/ Immunofluorescence: Methyl-Lysine Antibody [NB600-824]

Immunocytochemistry/Immunofluorescence: Methyl-Lysine Antibody [NB600-824] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Methylated Lysine Polyclonal Antibody at 1:50 for 12 hours at 4 degrees C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Methylated Lysine Antibody. (C) Composite.

Applications for Methyl-Lysine Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:200

Immunoprecipitation

1:10-1:500

Western Blot

1:2000-1:5000
Application Notes
0.2-0.5 ug/mL of Methyl-Lysine Antibody was sufficient for detection of the methylated histone by Western blot analysis using melanoma cells in TBSt.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 50% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 °C.

Background: Methyl-Lysine

Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves methylation (1). Lysine can be methylated once, twice or three times by lysine methyltransferases. The transfer of methyl groups from S-adenosyl methionine to histones is catalyzed by enzymes known as histone methyltransferases. Histones which are methylated on certain residues can act epigenetically to repress or activate gene expression (1, 2). The transcriptional repressor SUV39H1 can encode novel enzymes which selectively methylate histone H3 at lysine 9. SUV39H1 places a methyl marker on histone H3, which is then recognized by HP1 through its chromo domain. This model may also explain the stable inheritance of the heterochromatic state (3). Some studies have also speculated a stimulatory role for transcription by methylated histone lyside 4 due to its presence at active transcription sites (4-6).

Alternate Names

Dimethylysine, Methyl lysine, methyllysine, monomethyl lysine, N epsilon dimethyl lysine, N epsilon mono methyllysine, N epsilon trimethyl lysine, N(epsilon) trimethyl lysine, Trimethyl lysine

Additional Methyl-Lysine Products

Product Documents for Methyl-Lysine Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Methyl-Lysine Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Methyl-Lysine Antibody - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Methyl-Lysine Antibody - Azide and BSA Free

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  • Q: I am interested in your anti-methylated lysine antibody (catalog # NB600-824). Could you kindly tell me which type of methylated lysine is recognized by the antibody, monomethyl, dimethyl or trimethyl, or all.

    A: This product recognizes mono-methyl and di-methyl, but has not yet been tested for tri-methyl.

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