METT10D Antibody (OTI3E8)
Novus Biologicals | Catalog # NBP2-45823
Key Product Details
Species Reactivity
Human, Mouse, Canine
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI3E8
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Product Specifications
Immunogen
Full length human recombinant protein of human METT10D(NP_076991) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
63.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for METT10D Antibody (OTI3E8)
![Western Blot: METT10D Antibody (OTI3E8) [NBP2-45823]](https://resources.rndsystems.com/images/products/METT10D-Antibody-3E8-Western-Blot-NBP2-45823-img0004.jpg "Western Blot: METT10D Antibody (OTI3E8) [NBP2-45823]")
Western Blot: METT10D Antibody (OTI3E8) [NBP2-45823]
Western Blot: METT10D Antibody (3E8) [NBP2-45823] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY METT10D.![Immunohistochemistry: METT10D Antibody (OTI3E8) [NBP2-45823]](https://resources.rndsystems.com/images/products/METT10D-Antibody-3E8-Immunohistochemistry-NBP2-45823-img0003.jpg "Immunohistochemistry: METT10D Antibody (OTI3E8) [NBP2-45823]")
Immunohistochemistry: METT10D Antibody (OTI3E8) [NBP2-45823]
Immunohistochemistry: METT10D Antibody (3E8) [NBP2-45823] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Western Blot: METT10D Antibody (OTI3E8) [NBP2-45823]](https://resources.rndsystems.com/images/products/METT10D-Antibody-3E8-Western-Blot-NBP2-45823-img0005.jpg "Western Blot: METT10D Antibody (OTI3E8) [NBP2-45823]")
Western Blot: METT10D Antibody (OTI3E8) [NBP2-45823]
Western Blot: METT10D Antibody (3E8) [NBP2-45823] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).![Immunohistochemistry: METT10D Antibody (OTI3E8) [NBP2-45823]](https://resources.rndsystems.com/images/products/METT10D-Antibody-3E8-Immunohistochemistry-NBP2-45823-img0001.jpg "Immunohistochemistry: METT10D Antibody (OTI3E8) [NBP2-45823]")
Immunohistochemistry: METT10D Antibody (OTI3E8) [NBP2-45823]
Immunohistochemistry: METT10D Antibody (3E8) [NBP2-45823] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: METT10D Antibody (OTI3E8) [NBP2-45823]](https://resources.rndsystems.com/images/products/METT10D-Antibody-3E8-Immunohistochemistry-NBP2-45823-img0002.jpg "Immunohistochemistry: METT10D Antibody (OTI3E8) [NBP2-45823]")
Immunohistochemistry: METT10D Antibody (OTI3E8) [NBP2-45823]
Immunohistochemistry: METT10D Antibody (3E8) [NBP2-45823] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)Applications for METT10D Antibody (OTI3E8)
Application
Recommended Usage
Immunohistochemistry
1:500
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: METT10D
Alternate Names
EC 2.1.1.-, methyltransferase 10 domain containing, Methyltransferase 10 domain-containing protein, methyltransferase like 16, METT10D, MGC3329, putative methyltransferase METT10D
Gene Symbol
METTL16
Additional METT10D Products
Product Documents for METT10D Antibody (OTI3E8)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for METT10D Antibody (OTI3E8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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