![Immunohistochemistry-Paraffin: METTL21D Antibody [NBP1-93526]](https://resources.rndsystems.com/images/products/METTL21D-Antibody-Immunohistochemistry-Paraffin-NBP1-93526-img0004.jpg "Immunohistochemistry-Paraffin: METTL21D Antibody [NBP1-93526]")
Loading...
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DTLESSLEDPLRSFVRVLEKRDGTVLRLQQYSSGGVGCVVWDAAIVLSKYLETPEFSGDGAHALSRRSVLELGSGTGAVGLMAATLGADVVVTDLEELQDLLKMNINMNKHLVTGSVQAKGGRNRRLSFSTRLHTDGRLHI
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for METTL21D Antibody - BSA Free
Immunohistochemistry-Paraffin: METTL21D Antibody [NBP1-93526]
Immunohistochemistry-Paraffin: METTL21D Antibody [NBP1-93526] - Staining of human breast shows moderate cytoplasmic positivity in glandular cells.![METTL21D Antibody - BSA Free Immunohistochemistry: METTL21D Antibody - BSA Free [NBP1-93526]](https://resources.rndsystems.com/images/products/nbp1-93526_rabbit-polyclonal-mettl21d-antibody-29520251643588.jpg "Immunohistochemistry: METTL21D Antibody - BSA Free [NBP1-93526]")
Immunohistochemistry: METTL21D Antibody - BSA Free [NBP1-93526]
Staining of human fallopian tube shows weak cytoplasmic positivity in glandular cells.![METTL21D Antibody - BSA Free Immunohistochemistry: METTL21D Antibody - BSA Free [NBP1-93526]](https://resources.rndsystems.com/images/products/nbp1-93526_rabbit-polyclonal-mettl21d-antibody-30520257483215.jpg "Immunohistochemistry: METTL21D Antibody - BSA Free [NBP1-93526]")
Immunohistochemistry: METTL21D Antibody - BSA Free [NBP1-93526]
Staining of human testis shows strong cytoplasmic positivity in Leydig cells.![METTL21D Antibody - BSA Free Immunohistochemistry: METTL21D Antibody - BSA Free [NBP1-93526]](https://resources.rndsystems.com/images/products/nbp1-93526_rabbit-polyclonal-mettl21d-antibody-295202516321318.jpg "Immunohistochemistry: METTL21D Antibody - BSA Free [NBP1-93526]")
Immunohistochemistry: METTL21D Antibody - BSA Free [NBP1-93526]
Staining of human pancreas shows very weak cytoplasmic positivity in exocrine glandular cells.![METTL21D Antibody - BSA Free Immunohistochemistry: METTL21D Antibody - BSA Free [NBP1-93526]](https://resources.rndsystems.com/images/products/nbp1-93526_rabbit-polyclonal-mettl21d-antibody-30520259595910.jpg "Immunohistochemistry: METTL21D Antibody - BSA Free [NBP1-93526]")
Immunohistochemistry: METTL21D Antibody - BSA Free [NBP1-93526]
Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.Applications for METTL21D Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Reviewed Applications
Read 1 review rated 1 using NBP1-93526 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: METTL21D
Alternate Names
C14orf138, FLJ13920, methyltransferase like 21D, methyltransferase-like protein 21D
Gene Symbol
VCPKMT
Additional METTL21D Products
Product Documents for METTL21D Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for METTL21D Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for METTL21D Antibody - BSA Free (1)
1 out of 5
1 Customer Rating
Have you used METTL21D Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Customer Images
Showing
1
-
1 of
1 review
Showing All
Filter By:
-
Application: Western BlotSample Tested: 293T whole cell lysate, 293T cells lysate and 293T cell lysateSpecies: HumanVerified Customer | Posted 10/02/2018antibody 1:1000 dilution overnight 4C incubation. Secondary staining was performed with protein A/G-HRP Pierce, 1:10000 dilution. blocking/incubation: 5% milk TBS with 0.1% tween;. Exposure of 800 seconds - METTL21D size 25.8kDa.used in 1:1000 dilution in 5% milk TBS 0.1% tween, overnight 4C incubation. exposure 800 seconds
There are no reviews that match your criteria.
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...