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Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 400 and 450 of human Mahogunin, Ring Finger 1 using the numbering given in entry NP_001135761.1 (GeneID 23295).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MGRN1 Antibody
Western Blot: MGRN1 Antibody [NBP1-49929]
Western Blot: MGRN1 Antibody [NBP1-49929] - Detection of Human MGRN1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-MGRN1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. MGRN1 was also immunoprecipitated by rabbit anti-MGRN1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated MGRN1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).Applications for MGRN1 Antibody
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MGRN1
Alternate Names
E3 ubiquitin-protein ligase MGRN1, EC 6.3.2, KIAA0544probable E3 ubiquitin-protein ligase MGRN1, Mahogunin RING finger protein 1, mahogunin, ring finger 1, RING finger protein 156, RNF156EC 6.3.2.-
Gene Symbol
MGRN1
Additional MGRN1 Products
Product Documents for MGRN1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MGRN1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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