MICA Antibody (9F1) - BSA Free

Novus Biologicals | Catalog # NBP3-26140

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9F1 expressed in HEK293

Format

BSA Free
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Product Specifications

Immunogen

A synthesized peptide derived from Human MICA [UniProt Q29983]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MICA Antibody (9F1) - BSA Free

MICA Antibody (9F1)

Immunohistochemistry: MICA Antibody (9F1) [NBP3-26140] -

Immunohistochemistry: MICA Antibody (9F1) [NBP3-26140] - Image of MICA Antibody (9F1) diluted at 1:80 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.49% DAB.
MICA Antibody (9F1)

Immunohistochemistry: MICA Antibody (9F1) [NBP3-26140] -

Immunohistochemistry: MICA Antibody (9F1) [NBP3-26140] - Image of MICA Antibody (9F1) diluted at 1:80 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.49% DAB.

Applications for MICA Antibody (9F1) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: MICA

MICA encodes the higly polymorphic MHC (HLA) class I chain-related gene A. The protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells.

Long Name

MHC Class I-related Protein A

Alternate Names

FLJ60820, MGC111087, PERB11.1

Gene Symbol

MICA

Additional MICA Products

Product Documents for MICA Antibody (9F1) - BSA Free

Certificate of Analysis

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Product Specific Notices for MICA Antibody (9F1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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