MID1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-05061

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MID1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 478-667 of human MID1 (NP_000372.1). KLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MID1 Antibody - BSA Free

Western Blot: MID1 AntibodyBSA Free [NBP3-05061]

Western Blot: MID1 AntibodyBSA Free [NBP3-05061]

Western Blot: MID1 Antibody [NBP3-05061] - analysis of extracts of HeLa cells, using MID1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.
Immunocytochemistry/ Immunofluorescence: MID1 Antibody - BSA Free [NBP3-05061]

Immunocytochemistry/ Immunofluorescence: MID1 Antibody - BSA Free [NBP3-05061]

Immunocytochemistry/Immunofluorescence: MID1 Antibody [NBP3-05061] - Analysis of MCF-7 cells using MID1 antibody. Blue: DAPI for nuclear staining.

Applications for MID1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MID1

Defects in the Midline-1 (MID1) gene are the cause of Opitz syndrome, an X-linked recessive disorder characterized by developmental defects. MID1 is a microtubule-associated protein (MAP) with E3 ubiquitin ligase activity. MID1 has been shown to target protein phosphatases 2A (PP2Ac) on microtubules for degradation. MID1 association with PP2A has been proposed to regulate microtubule stabilization for cellular processes such as cell division and migration.

Alternate Names

EC 6.3.2.-, FLJ57031, FLJ58683, FLJ76288, FXYGBBB1, Midin, midline 1 (Opitz/BBB syndrome), RNF59MIDIN, TRIM18mouse, homolog of

Gene Symbol

MID1

Additional MID1 Products

Product Documents for MID1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MID1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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