MIG2/Kindlin-2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-87884
![MIG2/Kindlin-2 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: MIG2/Kindlin-2 Antibody - BSA Free [NBP1-87884]](https://resources.rndsystems.com/images/products/nbp1-87884_rabbit-polyclonal-mig2-kindlin-2-antibody-6520251627481.jpg "Immunocytochemistry/ Immunofluorescence: MIG2/Kindlin-2 Antibody - BSA Free [NBP1-87884]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (94%), Rat (97%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MADSSYNLEVQNILSFLKMQHLNPDPQLIPEQITTDITPECLVSPRYLKKYKNKQITARILEA
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MIG2/Kindlin-2 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: MIG2/Kindlin-2 Antibody - BSA Free [NBP1-87884]
Staining of human cell line U-2 OS shows localization to nucleoplasm & focal adhesion sites.![MIG2/Kindlin-2 Antibody - BSA Free Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody - BSA Free [NBP1-87884]](https://resources.rndsystems.com/images/products/nbp1-87884_rabbit-polyclonal-mig2-kindlin-2-antibody-6520251620320.jpg "Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody - BSA Free [NBP1-87884]")
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody - BSA Free [NBP1-87884]
Staining of human colon shows strong cytoplasmic positivity in lymphoid cells.![MIG2/Kindlin-2 Antibody - BSA Free Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody - BSA Free [NBP1-87884]](https://resources.rndsystems.com/images/products/nbp1-87884_rabbit-polyclonal-mig2-kindlin-2-antibody-65202516164210.jpg "Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody - BSA Free [NBP1-87884]")
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody - BSA Free [NBP1-87884]
Staining of human skin shows very weak cytoplasmic positivity in squamous epithelial cells.![MIG2/Kindlin-2 Antibody - BSA Free Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody - BSA Free [NBP1-87884]](https://resources.rndsystems.com/images/products/nbp1-87884_rabbit-polyclonal-mig2-kindlin-2-antibody-652025162161.jpg "Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody - BSA Free [NBP1-87884]")
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody - BSA Free [NBP1-87884]
Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.![MIG2/Kindlin-2 Antibody - BSA Free Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody - BSA Free [NBP1-87884]](https://resources.rndsystems.com/images/products/nbp1-87884_rabbit-polyclonal-mig2-kindlin-2-antibody-65202516221021.jpg "Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody - BSA Free [NBP1-87884]")
Immunohistochemistry-Paraffin: MIG2/Kindlin-2 Antibody - BSA Free [NBP1-87884]
Staining of human placenta shows strong cytoplasmic positivity in endothelial cells.![MIG2/Kindlin-2 Antibody - BSA Free Western Blot: MIG2/Kindlin-2 Antibody - BSA Free [NBP1-87884]](https://resources.rndsystems.com/images/products/nbp1-87884_rabbit-polyclonal-mig2-kindlin-2-antibody-6520251628556.jpg "Western Blot: MIG2/Kindlin-2 Antibody - BSA Free [NBP1-87884]")
Western Blot: MIG2/Kindlin-2 Antibody - BSA Free [NBP1-87884]
Analysis in human cell line SiHa.Applications for MIG2/Kindlin-2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MIG2
Long Name
Mitogen Inducible Gene 2
Alternate Names
FERMT2, KIND2, Kindlin-2, PLEKHC1
Entrez Gene IDs
10979 (Human)
Gene Symbol
FERMT2
UniProt
Additional MIG2 Products
Product Documents for MIG2/Kindlin-2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MIG2/Kindlin-2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for MIG2/Kindlin-2 Antibody - BSA Free
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Q: I bought an antibody named kindlin-2(NBP1-87884) for IHC a few days ago. I want to know the antigen retrieval condition your lab has employed.
A:
Our lab used a heat mediated citrate buffer pH 6.0 antigen retrieval method that can be found in our antigen retrieval protocol.
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