Minocycline hydrochloride

Tocris Bioscience | Catalog # 3268

Antibiotic. Displays neuroprotective effects
Tocris Bioscience

Key Product Details

Description

Antibiotic. Displays neuroprotective effects
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Product Description

Minocycline hydrochloride is a tetracycline antibiotic. Displays neuroprotective, antiapoptotic, anti-inflammatory and antimicrobial effects. Acts as a matrix metalloproteinase (MMP) inhibitor; attenuates disease severity in mouse models of multiple sclerosis. Orally active and brain penetrant. Allows formation of extended pluripotent stem (EPS) cells in combination with CHIR 99021 (Cat.No. 4423), (S)-(+)-Dimethindene maleate (Cat.No. 1425) and human leukemia inhibitory factor.

For more information about how Minocycline hydrochloride may be used, see our protocol: Generation and Propagation of EPS cells (LCDM Cocktail).

Product Specifications for Minocycline hydrochloride

Molecular Weight

493.94

Formula

C23H27N3O7.HCl

Storage

Store at +4°C

Purity

≥98% (HPLC)

Chemical Name

[4S-(4α,4aα,5aα,12aα)]-4,7-Bis(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,10,12,12a,tetrahydroxy-1,11-dioxo-2-naphthacenecarboxamide

CAS Number

13614-98-7

PubChem ID

54685925

InChI Key

WTJXVDPDEQKTCV-VQAITOIOSA-N

SMILES

NC(C4=C(O)[C@@H](N(C)C)[C@@]3([H])[C@](C4=O)(O)C(O)=C2C(C1=C(O)C=CC(N(C)C)=C1C[C@]([H])2C3)=O)=O.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 12.35 25
DMSO 49.39 100

Preparing Stock Solutions for Minocycline hydrochloride

The following data is based on the product molecular weight 493.94.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.02 mL 10.12 mL 20.25 mL
5 mM 0.40 mL 2.02 mL 4.05 mL
10 mM 0.20 mL 1.01 mL 2.02 mL
50 mM 0.04 mL 0.20 mL 0.40 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 20 publications citing the usage of this product.

Product Documents for Minocycline hydrochloride

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Minocycline hydrochloride

For research use only

⚠ WARNING: This product can expose you to chemicals including Minocycline hydrochloride (internal use), which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Citations for Minocycline hydrochloride

Customer Reviews for Minocycline hydrochloride (1)

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  • Blocker of microglia reactivity
    Name: Luiz Alexandre Magno
    Species: Mouse
    Assay Type: In Vivo
    Cell Line/Tissue: Brain
    Verified Customer | Posted 07/16/2018
    I have used the antibiotic minocycline to impair activation of microglia in the brain of mice. This drug has anti-inflammatory properties that block microglial reactivity, which can be easily confirmed by expression of the CD68 protein. The drug was diluted in PBS and injected i.p. (75 mg/kg) in mice, twice a day, for 7 days. The image shows that microglial cells (stained for Iba1) do not express CD68 (in red) after treatment with minocycline.
    Minocycline hydrochloride 3268

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