MitoBrilliant™ Live 646
Tocris Bioscience | Catalog # 7417
Key Product Details
Description
Wavelength
Product Description
MitoBrilliant probes have been developed to overcome some common limitations encountered with standard mitochondrial trackers, offering clearer answers to scientific questions. They are next-generation fluorescent stains for the localization and tracking of mitochondria in both live and fixed cells. The range harnesses Janelia Fluor® dye technology, conferring some of the properties that make these widely used dyes, into mitochondrial stains.
Key information: MitoBrilliant™ Live 646 is a red-fluorescent mitochondrial stain for live cells, Δψm dependent.
Used for: mitochondrial staining for live cells.
Application: flow cytometry, high content screening.
Properties and Photophysical Data: The MitoBrilliant™ Live dyes accumulate in the mitochondria of live cells in a mitochondrial membrane potential (Δψm) dependent manner. Upon loss of the mitochondrial membrane potential, the dyes disperse, and so provide a dynamic assessment of the mitochondrial membrane potential in live cells. Excitation and emission maxima (λ) are 648 nm and 662 nm, respectively, extinction coefficient = 127,800 M-1cm-1.
Two MitoBrilliant™ Live dyes are available: MitoBrilliant™ Live 646 (red emission) and MitoBrilliant™ Live 549 (yellow/orange emission).
A universal stain for mitochondria in both live and fixed cells is also available: MitoBrilliant™ 646.
Please refer to the protocol for guidelines on product use, and download the MitoBrilliant Product Guide to view data for each of the dyes in different applications.
MitoBrilliant™ is a trademark of Bio-Techne Corp.
Scientific Data Images for MitoBrilliant™ Live 646
Live-cell image of mitochondria stained with MitoBrilliant™ Live 646
HeLa cells were incubated with MitoBrilliant™ Live 646 (100 nM) for 40 minutes and counter stained with DAPI (Catalog # 5748). Image was taken using an LSM880 Confocal using a 63x objective. Scale bar = 10 µm.
| Product Name | Core dye structure | Abs/Em (nm) | Δψm dependent | Live/Fixed cell use | Image without wash step | Demonstrated Applications |
MitoBrilliant™ 646 Cat. No. 7700 |
Janelia Fluor® technology | 655/668 | No* | Suitable for both live and fixed cell work | Yes, but replacing media recommended |
Fixed-cell imaging, Live-cell imaging, Flow Cytometry, IHC/ICC, Super-resolution microscopy – STED, High-content screening |
|---|---|---|---|---|---|---|
|
MitoBrilliant™ Live 646 Cat. No. 7417 |
Janelia Fluor® technology | 648/662 | Yes | Live-cell work only | Yes, but replacing media recommended |
Live-cell imaging, Flow Cytometry, High-content screening |
|
MitoBrilliant™ Live 549 Cat. No. 7693 |
Janelia Fluor® technology | 550/568 | Yes | Live-cell work only | Yes, but replacing media recommended |
Live-cell imaging, Flow Cytometry, High-content screening |
Optical Data
| Emission Color | Red |
| λabs | 648 nm |
| λem | 662 nm |
| Extinction Coefficient (ε) | 127800 M-1cm-1 |
| Closest Laser line | 640 nm |
| Application | Live-cell Imaging, Flow Cytometry, High-content Screening |
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Product Specifications for MitoBrilliant™ Live 646
Molecular Weight
Storage
Purity
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 8.9 | 20 |
Preparing Stock Solutions for MitoBrilliant™ Live 646
The following data is based on the product molecular weight 445.08.
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 11.23 mL | 56.17 mL | 112.34 mL |
| 1 mM | 2.25 mL | 11.23 mL | 22.47 mL |
| 2 mM | 1.12 mL | 5.62 mL | 11.23 mL |
| 10 mM | 0.22 mL | 1.12 mL | 2.25 mL |
Calculators
Background References
References are publications that support the biological activity of the product.
There are currently no references for this product.
Product Documents for MitoBrilliant™ Live 646
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MitoBrilliant™ Live 646
For research use only
Citations for MitoBrilliant™ Live 646
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