MitoBrilliant™ 646

Catalog # Availability Size / Price Qty
7700/1 PACK
MitoBrilliant™ 646 | Mitochondria Dyes and Probes
2 Images
Description: Universal red fluorescent mitochondrial stain for both live and fixed cells

Purity: ≥95%

Product Details

Biological Activity

MitoBrilliant probes have been developed to overcome some common limitations encountered with standard mitochondrial trackers, offering clearer answers to scientific questions. They are next-generation fluorescent stains for the localization and tracking of mitochondria in both live and fixed cells. The range harnesses Janelia Fluor® dye technology, conferring some of the properties that make these widely used dyes, into mitochondrial stains.

MitoBrilliant™ 646 is a next-generation universal stain for mitochondria in both live and fixed cells. It is retained in mitochondria following fixation with exceptionally clear staining and is retained in the mitochondria of live cells following loss of the mitochondrial membrane potential. Additionally, MitoBrilliant™ 646 can be used to stain pre-fixed cells/tissue, making this dye suitable for IHC/ICC.

Two dyes that accumulate in the mitochondria of live cells in a mitochondrial membrane potential (Δψm) dependent manner are also available: MitoBrilliant™ Live 646 (red emission) and MitoBrilliant™ Live 549 (yellow/orange emission).

Please refer to the protocol for guidelines on product use, and download the MitoBrilliant Product Guide to view data for each of the dyes in different applications.

MitoBrilliant is a trademark of Bio-Techne Corp.

Scientific Data

Live-cell image of mitochondria stained with MitoBrilliant™ 646 HeLa cells were incubated with MitoBrilliant™ 646 (100 nM) for 40 minutes and counterstained with DAPI (Catalog # 5748). Image was taken using an LSM880 Confocal using a 63x objective. Scale bar = 10 µm.

Product Name Core dye structure Abs/Em (nm) Δψm dependent Live/Fixed cell use Stain pre-fixed cells? Image without wash step Demonstrated Applications
MitoBrilliant™ 646
Cat. No. 7700
Janelia Fluor® technology 655/668 No* Suitable for both live and fixed cell work Yes Yes, but replacing media recommended Fixed-cell imaging,
Live-cell imaging,
Flow Cytometry,
Super-resolution microscopy – STED,
High-content screening
MitoBrilliant™ Live 646
Cat. No. 7417
Janelia Fluor® technology 648/662 Yes Live-cell work only No Yes, but replacing media recommended Live-cell imaging,
Flow Cytometry,
High-content screening
MitoBrilliant™ Live 549
Cat. No. 7693
Janelia Fluor® technology 550/568 Yes Live-cell work only No Yes, but replacing media recommended Live-cell imaging,
Flow Cytometry,
High-content screening

Optical Data for MitoBrilliant™ 646

MitoBrilliant™ 646 Dye Spectra
Emission Color Red
λabs 655 nm
λem 668 nm
Extinction Coefficient (ε) 125000 M-1cm-1
Closest Laser line 640 nm

Technical Data

Soluble to 10 mM in DMSO
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Product Datasheets

Reconstitution Calculator
Molarity Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Molarity Calculator


*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).


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Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.