Mitofilin Antibody (M027)

Novus Biologicals | Catalog # NBP3-23409

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M027
View all Mitofilin Primary Antibodies »

Product Specifications

Immunogen

Clone M027 was generated from a proprietary antigen related to human mitofilin expressed in the A431 epidermoid carcinoma cell line. [UniProt# Q16891]

Specificity

Clone M027 detects a 90 kDa band corresponding to the molecular mass of mitofilin on SDS-PAGE immunoblots of native or denatured human PC3, MDA-MB-231, A431, A549, and MeWo cell lysates, as well as human breast, skin, and brain tissues. In addition, mass spectrometry analysis of immunoprecipitates using MM0271 in human A431 cell lysates confirms this antibody only detects Mitofilin protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Mitofilin Antibody (M027)

Mitofilin Antibody (M027)

Immunocytochemistry/Immunofluorescence: Mitofilin Antibody (M027) [NBP3-23409] -

Immunocytochemistry/Immunofluorescence: Mitofilin Antibody (M027) [NBP3-23409] - Immunocytochemical labeling of mitofilin in methanol-acetone (1:1) fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-mitofilin (clone M027). The antibody was detected using goat anti-mouse DyLight(R) 594.
Mitofilin Antibody (M027)

Immunocytochemistry/Immunofluorescence: Mitofilin Antibody (M027) [NBP3-23409] -

Immunocytochemistry/Immunofluorescence: Mitofilin Antibody (M027) [NBP3-23409] - Immunocytochemical labeling of mitofilin in methanol-acetone (1:1) fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-mitofilin (clone M027). The antibody was detected using goat anti-mouse DyLight(R) 594.
Mitofilin Antibody (M027)

Western Blot: Mitofilin Antibody (M027) [NBP3-23409] -

Western Blot: Mitofilin Antibody (M027) [NBP3-23409] - Western blot analysis of human cell lysates: MeWo (lane 1), MDA-MB-231 (lane 2), PC3 (lane 3), and A549 (lane 4). The blot was probed with mouse monoclonal anti-mitofilin (NBP3-23409) at 1:1000.

Applications for Mitofilin Antibody (M027)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunoprecipitation

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Mitofilin

Mitochondria are the center of cellular energy production and essential metabolic reactions. As double membrane-bound organelles, mitochondria from different species, tissues, and metabolic states are highly polymorphic in nature, yet exhibit common structural features. The ultrastructural variations in mitochondrial architecture occur mainly due to the differences in the amount and shape of cristae. Abundant cristae are found in mitochondria from tissues where energy demand is high. Analysis of the human heart mitochondrial proteome shows that mitofilin is one of the most abundant mitochondrial proteins. It appears to play an important role in the maintenance of cristae morphology. Mitofilin was originally described as heart muscle protein (HMP) because of its high expression in the heart.

Alternate Names

DKFZp779P1653, inner membrane protein, mitochondrial, MGC111146, Mic 60, Mic60, MICOS Complex Subunit MIC60, mitochondrial (mitofilin), Mitofilin, motor protein, p87/89, proliferation-inducing gene 4

Gene Symbol

IMMT

Additional Mitofilin Products

Product Documents for Mitofilin Antibody (M027)

Certificate of Analysis

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Product Specific Notices for Mitofilin Antibody (M027)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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