MKK3/MEK3 Antibody (1D10) - Azide and BSA Free

Name: MKK3/MEK3 Antibody (1D10) - Azide and BSA Free
Brand: Novus Biologicals
SKU: H00005606-M02
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # H00005606-M02

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 1D10

Format

Azide and BSA Free

View all MKK3 Primary Antibodies »

Product Specifications

Immunogen

MAP2K3 (AAH32478, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MESPASSQPASMPQSKGKSKRKKDLRISCMSKPPAPNPTPPRNLDSRTFITIGDRNFEVEADDLVTISELGRGAYGVVEKVRHAQSGTIMAVKRIRATVN

Localization

Secreted

Specificity

MAP2K3 - mitogen-activated protein kinase kinase 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MKK3/MEK3 Antibody (1D10) - Azide and BSA Free

Western Blot: MKK3/MEK3 Antibody (1D10) [H00005606-M02]

Western Blot: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Analysis of MAP2K3 expression in transfected 293T cell line by MAP2K3 monoclonal antibody (M02), clone 1D10.Lane 1: MAP2K3 transfected lysate (Predicted MW: 39.3 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: MKK3/MEK3 Antibody (1D10) [H00005606-M02]

Immunohistochemistry-Paraffin: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Analysis of monoclonal antibody to MAP2K3 on formalin-fixed paraffin-embedded human dysgerminoma. Antibody concentration 3 ug/ml.

Western Blot: MKK3/MEK3 Antibody (1D10) [H00005606-M02]

Western Blot: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Western Blot analysis of MAP2K3 expression in Hela NE ( Cat # L013V3 ).

Immunoprecipitation: MKK3/MEK3 Antibody (1D10) [H00005606-M02]

Immunoprecipitation: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Analysis of MAP2K3 transfected lysate using anti-MAP2K3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAP2K3 monoclonal antibody.

Sandwich ELISA: MKK3/MEK3 Antibody (1D10) [H00005606-M02] - Detection limit for recombinant GST tagged MAP2K3 is approximately 0.03ng/ml as a capture antibody.

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Applications for MKK3/MEK3 Antibody (1D10) - Azide and BSA Free

Application

Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Sandwich ELISA

1:100-1:2000

Western Blot

1:500

Application Notes

Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MKK3

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene.

Long Name

Mitogen-activated Protein Kinase Kinase 3

Alternate Names

MAP2K3, MEK3, PRKMK3

Entrez Gene IDs

5606 (Human)

Gene Symbol

MAP2K3

OMIM

602315 (Human)

UniProt

AAH32478 (Human)

Additional MKK3 Products

Product Documents for MKK3/MEK3 Antibody (1D10) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for MKK3/MEK3 Antibody (1D10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.