MKK6/MEK6 Antibody (2F2) - Azide and BSA Free

Name: MKK6/MEK6 Antibody (2F2) - Azide and BSA Free
Brand: Novus Biologicals
SKU: H00005608-M02
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # H00005608-M02

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F2

Format

Azide and BSA Free

View all MKK6 Primary Antibodies »

Product Specifications

Immunogen

MAP2K6 (AAH12009, 231 a.a. ~ 334 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QKGYSVKSDIWSLGITMIELAILRFPYDSWGTPFQQLKQVVEEPSPQLPADKFSAEFVDFTSQCLKKNSKERPTYPELMQHPFFTLHESKGTDVASFVKLILGD

Specificity

MAP2K6 (2F2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MKK6/MEK6 Antibody (2F2) - Azide and BSA Free

Western Blot: MKK6/MEK6 Antibody (2F2) [H00005608-M02]

Western Blot: MKK6/MEK6 Antibody (2F2) [H00005608-M02] - MAP2K6 monoclonal antibody (M02), clone 2F2 Analysis of MAP2K6 expression in COLO 320 HSR.

Immunocytochemistry/ Immunofluorescence: MKK6/MEK6 Antibody (2F2) [H00005608-M02]

Immunocytochemistry/Immunofluorescence: MKK6/MEK6 Antibody (2F2) [H00005608-M02] - Analysis of monoclonal antibody to MAP2K6 on HeLa cell. Antibody concentration 10 ug/ml.

Proximity Ligation Assay: MKK6/MEK6 Antibody (2F2) [H00005608-M02]

Proximity Ligation Assay: MKK6/MEK6 Antibody (2F2) [H00005608-M02] - Analysis of protein-protein interactions between MAP2K3 and MAP2K6. HeLa cells were stained with anti-MAP2K3 rabbit purified polyclonal 1:1200 and anti-MAP2K6 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Sandwich ELISA: MKK6/MEK6 Antibody (2F2) [H00005608-M02] - Detection limit for recombinant GST tagged MAP2K6 is approximately 0.3ng/ml as a capture antibody.

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Applications for MKK6/MEK6 Antibody (2F2) - Azide and BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Sandwich ELISA

1:100-1:2000

Western Blot

1:500

Application Notes

Antibody reactivity against cell lysate for WB. It has been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MKK6

This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.

Long Name

Mitogen-activated Protein Kinase Kinase 6

Alternate Names

MAP2K6, MEK6, PRKMK6, SAPKK3

Entrez Gene IDs

5608 (Human)

Gene Symbol

MAP2K6

OMIM

601254 (Human)

UniProt

AAH12009 (Human)

Additional MKK6 Products

Product Documents for MKK6/MEK6 Antibody (2F2) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for MKK6/MEK6 Antibody (2F2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.