MKP-3/DUSP6 Antibody (1V5L10)

Novus Biologicals | Catalog # NBP3-15252

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1V5L10 expressed in HEK293
View all MKP-3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 282-381 of human MKP-3/DUSP6 (Q16828). ARGKNCGVLVHCLAGISRSVTVTVAYLMQKLNLSMNDAYDIVKMKKSNISPNFNFMGQLLDFERTLGLSSPCDNRVPAQQLYFTTPSNQNVYQVDSLQST

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit MKP-3/DUSP6 Antibody (1V5L10) (NBP3-15252) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MKP-3/DUSP6 Antibody (1V5L10)

Western Blot: MKP-3/DUSP6 Antibody (1V5L10) [NBP3-15252]

Western Blot: MKP-3/DUSP6 Antibody (1V5L10) [NBP3-15252]

Western Blot: MKP-3/DUSP6 Antibody (1V5L10) [NBP3-15252] - Analysis of extracts of various cell lines, using MKP-3/DUSP6 Rabbit mAb (NBP3-15252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: MKP-3/DUSP6 Antibody (1V5L10) [NBP3-15252]

Immunohistochemistry-Paraffin: MKP-3/DUSP6 Antibody (1V5L10) [NBP3-15252]

Immunohistochemistry-Paraffin: MKP-3/DUSP6 Antibody (1V5L10) [NBP3-15252] - Human colon using MKP-3/DUSP6 Rabbit mAb (NBP3-15252) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
MKP-3/DUSP6 Antibody (1V5L10)

Immunohistochemistry: MKP-3/DUSP6 Antibody (1V5L10) [MKP-3/DUSP6] -

Immunohistochemistry: MKP-3/DUSP6 Antibody (1V5L10) [MKP-3/DUSP6] - Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using MKP-3/DUSP6 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
MKP-3/DUSP6 Antibody (1V5L10)

Western Blot: MKP-3/DUSP6 Antibody (1V5L10) [MKP-3/DUSP6] -

Western Blot: MKP-3/DUSP6 Antibody (1V5L10) [MKP-3/DUSP6] - Western blot analysis of various lysates using MKP-3/DUSP6 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
MKP-3/DUSP6 Antibody (1V5L10)

Western Blot: MKP-3/DUSP6 Antibody (1V5L10) [MKP-3/DUSP6] -

Western Blot: MKP-3/DUSP6 Antibody (1V5L10) [MKP-3/DUSP6] - Western blot analysis of various lysates using MKP-3/DUSP6 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 45s.

Applications for MKP-3/DUSP6 Antibody (1V5L10)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MKP-3

DUSP6 is encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.

Long Name

MAP Kinase Phosphatase 3

Alternate Names

DUSP6, MKP3, PYST1

Gene Symbol

DUSP6

Additional MKP-3 Products

Product Documents for MKP-3/DUSP6 Antibody (1V5L10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MKP-3/DUSP6 Antibody (1V5L10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for MKP-3/DUSP6 Antibody (1V5L10)

There are currently no reviews for this product. Be the first to review MKP-3/DUSP6 Antibody (1V5L10) and earn rewards!

Have you used MKP-3/DUSP6 Antibody (1V5L10)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies