Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry, Simple Western, Flow Cytometry (Negative)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 954724

Format

Azide and BSA Free
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Product Specifications

Immunogen

Synthetic peptide from human MLKL phosphorylated at T357 [UniProt Q8NB16]

Modification

p Thr357

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

>90% by SDS PAGE

Scientific Data Images for MLKL [p Thr357] Antibody (954724) - Azide and BSA Free

Immunohistochemistry-Paraffin: MLKL [p Thr357] Antibody (954724) [MAB91871] - MLKL was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using MLKL Monoclonal Antibody at 5 ug/ml. Tissue was stained using Goat anti-Mouse IgG Secondary Antibody [HRP Polymer] (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in thymocytes.
Immunohistochemistry: MLKL [p Thr357] Antibody (954724) [MAB91871] - MLKL was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using MLKL Monoclonal Antibody at 5 ug/ml. Tissue was stained using Mouse IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm in cancer cells.

Applications for MLKL [p Thr357] Antibody (954724) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml
Application Notes
This MLKL [p Thr357] Antibody (954724) is validated for Simple Western from a verified customer review.

Reviewed Applications

Read 2 reviews rated 4.5 using MAB91871 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MLKL

Long Name

Mixed Lineage Kinase Domain-like

Alternate Names

FLJ34389, mixed lineage kinase domain-like, mixed lineage kinase domain-like protein

Gene Symbol

MLKL

Additional MLKL Products

Product Documents for MLKL [p Thr357] Antibody (954724) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MLKL [p Thr357] Antibody (954724) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MLKL [p Thr357] Antibody (954724) - Azide and BSA Free

Customer Reviews for MLKL [p Thr357] Antibody (954724) - Azide and BSA Free (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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Customer Images

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  • MLKL [p Thr357] Antibody (954724)
    Name: Anonymous
    Application: Simple Western
    Sample Tested: HT-29 human colon adenocarcinoma cell line and MC-38
    Species: Human and Mouse
    Verified Customer | Posted 10/16/2020
    pMLKL Ab
    MLKL [p Thr357] Antibody (954724) - Azide and BSA Free MAB91871
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • MLKL [p Thr357] Antibody (954724)
    Name: Taotao Zhang
    Application: Immunohistochemistry-Paraffin
    Sample Tested: FFPE
    Species: Human
    Verified Customer | Posted 10/09/2020
    20X
    MLKL [p Thr357] Antibody (954724) - Azide and BSA Free MAB91871

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Protocols

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FAQs for MLKL [p Thr357] Antibody (954724) - Azide and BSA Free

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  • Q: hello! we're working with MAB91871. the IF staining is looking pretty good, but occasionally we are finding bright specular artifacts. From the secondary controls we have narrow it down to being due to the presence of the primary - do y'all have any handling data on this product and how to minimize aggregates?

    A:   Here are some general suggestions to try and reduce aggregation background signal in your organoid IF staining with MAB91871. 

    • This is a purified antibody with no BSA, so it ought not to be an issue with buffer but its possible it's could be sticky.
      Do you use  0.05-0.1 % Triton X 100?
    • You might try adding NaCl to the blocking buffer/antibody diluent so that the final concentration is between 0.15 M and 0.6 M NaCl. The best NaCl concentration to use will have to be determined empirically, but this should help reduce ionic interactions and keep the AB soluble. 
    • If it's a blocking issue, you could of course increase the blocking buffer composition and/or concentration.
    • It's possible that high primary AB concentration is leading to non-specific binding and background staining, so you might try reducing the final concentration of the primary antibody used.

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