MLKL Antibody - Azide and BSA Free
Novus Biologicals | Catalog # H00197259-B01P
![Western Blot: MLKL Antibody [H00197259-B01P]](https://resources.rndsystems.com/images/products/MLKL-Antibody-Western-Blot-H00197259-B01P-img0004.jpg "Western Blot: MLKL Antibody [H00197259-B01P]")
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Mouse IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
MLKL (NP_689862.1, 1 a.a. - 471 a.a.) full-length human protein. MENLKHIITLGQVIHKRCEEMKYCKKQCRRLGHRVLGLIKPLEMLQDQGKRSVPSEKLTTAMNRFKAALEEANGEIEKFSNRSNICRFLTASQDKILFKDVNRKLSDVWKELSLLLQVEQRMPVSPISQGASWAQEDQQDADEDRRAFQMLRRDNEKIEASLRRLEINMKEIKETLRQYLPPKCMQEIPQEQIKEIKKEQLSGSPWILLRENEVSTLYKGEYHRAPVAIKVFKKLQAGSIAIVRQTFNKEIKTMKKFESPNILRIFGICIDETVTPPQFSIVMEYCELGTLRELLDREKDLTLGKRMVLVLGAARGLYRLHHSEAPELHGKIRSSNFLVTQGYQVKLAGFELRKTQTSMSLGTTREKTDRVKSTAYLSPQELEDVFYQYDVKSEIYSFGIVLWEIATGDIPFQGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK
Specificity
MLKL - mixed lineage kinase domain-like,
Clonality
Polyclonal
Host
Mouse
Isotype
IgG
Description
Novus Biologicals Mouse MLKL Antibody - Azide and BSA Free (H00197259-B01P) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MLKL Antibody - Azide and BSA Free
Western Blot: MLKL Antibody [H00197259-B01P]
Western Blot: MLKL Antibody [H00197259-B01P] - Analysis of MLKL expression in transfected 293T cell line by MLKL polyclonal antibody. Lane 1: MLKL transfected lysate(51.81 KDa). Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: MLKL Antibody [H00197259-B01P]](https://resources.rndsystems.com/images/products/MLKL-Antibody-Immunocytochemistry-Immunofluorescence-H00197259-B01P-img0003.jpg "Immunocytochemistry/ Immunofluorescence: MLKL Antibody [H00197259-B01P]")
Immunocytochemistry/ Immunofluorescence: MLKL Antibody [H00197259-B01P]
Immunocytochemistry/Immunofluorescence: MLKL Antibody [H00197259-B01P] - Analysis of purified antibody to MLKL on HeLa cell. (antibody concentration 10 ug/ml)![Western Blot: MLKL Antibody [H00197259-B01P]](https://resources.rndsystems.com/images/products/MLKL-Antibody-Western-Blot-H00197259-B01P-img0001.jpg "Western Blot: MLKL Antibody [H00197259-B01P]")
Western Blot: MLKL Antibody [H00197259-B01P]
Western Blot: MLKL Antibody [H00197259-B01P] - Analysis of MLKL expression in transfected 293T cell line by MLKL MaxPab polyclonal antibody. Lane 1: MLKL transfected lysate (51.81 KDa). Lane 2: Non-transfected lysate.Applications for MLKL Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Western Blot
1:500
Application Notes
Antibody reactive against Recombinant Protein with GST tag on Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. It has also been used for immunofluorescence.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: MLKL
Long Name
Mixed Lineage Kinase Domain-like
Alternate Names
FLJ34389, mixed lineage kinase domain-like, mixed lineage kinase domain-like protein
Entrez Gene IDs
197259 (Human)
Gene Symbol
MLKL
UniProt
Additional MLKL Products
Product Documents for MLKL Antibody - Azide and BSA Free
Product Specific Notices for MLKL Antibody - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for MLKL Antibody - Azide and BSA Free
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A: Thank you for your patience while I waited to hear back from Abnova. They have informed me that for product H00197259-B01P, although the amount supplied is constant (50 ug per vial), the volume can vary between batches due to differences in concentration. The concentration can be 0.1-1 mg/ml so the typical volume is between 50-500 ul.
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