MMP-9 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-57940

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Sheep

Cited:

Human, Mouse, Rat, Ovine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MMP-9 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to the N-terminal of the human MMP-9 protein (between amino acids 150-200) UniProt P14780.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MMP-9 Antibody - BSA Free

Western Blot: MMP-9 AntibodyBSA Free [NBP1-57940]

Western Blot: MMP-9 AntibodyBSA Free [NBP1-57940]

Western Blot: MMP-9 Antibody [NBP1-57940] - Western blot of MMP-9. Image from verified customer review.
Immunohistochemistry-Paraffin: MMP-9 Antibody - BSA Free [NBP1-57940]

Immunohistochemistry-Paraffin: MMP-9 Antibody - BSA Free [NBP1-57940]

Immunohistochemistry-Paraffin: MMP-9 Antibody [NBP1-57940] - Human prostate cancer was detected using HRP/AEC red color stain. Dilution: 2-15 ug/mL.
Western Blot: MMP-9 AntibodyBSA Free [NBP1-57940]

Western Blot: MMP-9 AntibodyBSA Free [NBP1-57940]

Western Blot: MMP-9 Antibody [NBP1-57940] - Analysis of MMP-9 in mouse brain tumor lysate (GL261 implanted mouse glioma tissue, 40 ug/lane) using anti-MMP-9 antibody. Image from verified customer review.
Western Blot: MMP-9 AntibodyBSA Free [NBP1-57940]

Western Blot: MMP-9 AntibodyBSA Free [NBP1-57940]

Western Blot: MMP-9 Antibody [NBP1-57940] - HeLa cell lysate. Antibody at 1.25 ug/mL.
Western Blot: MMP-9 AntibodyBSA Free [NBP1-57940]

Western Blot: MMP-9 AntibodyBSA Free [NBP1-57940]

Western Blot: MMP-9 Antibody [NBP1-57940] - Human Fetal Lung. Antibody at 1.0 ug/mL.

Applications for MMP-9 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:250

Western Blot

1.25 ug/mL

Reviewed Applications

Read 4 reviews rated 4.3 using NBP1-57940 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MMP-9

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

Long Name

Matrix Metalloproteinase 9

Alternate Names

CLG4B, Gelatinase B, GELB, MANDP2, MMP9

Entrez Gene IDs

4318 (Human)

Gene Symbol

MMP9

UniProt

P14780

Additional MMP-9 Products

Product Documents for MMP-9 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MMP-9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MMP-9 Antibody - BSA Free

Customer Reviews for MMP-9 Antibody - BSA Free (4)

4.3 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
75%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • MMP-9 Antibody
    Name: Emily Thompson
    Application: Western Blot
    Sample Tested: conditioned media from human glioma cells and conditioned media from U251 human glioma cell line
    Species: Human
    Verified Customer | Posted 12/13/2019
    Primary antibody used at 1.25ug/ml on conditioned media from U251 human glioma cell lines
    MMP-9 Antibody - BSA Free NBP1-57940
  • MMP-9 Antibody
    Name: Emily Thompson
    Application: Western Blot
    Sample Tested: concentrated conditioned media and U251 glioma cell line; concentrated conditioned media
    Species: Human
    Verified Customer | Posted 05/02/2019
    Observed band ~67kDa; active MMP9
    MMP-9 Antibody - BSA Free NBP1-57940
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: GL261 implanted mouse glioma tissue
    Species: Mouse
    Verified Customer | Posted 12/12/2014
    MMP-9 Mouse Brain Tumor Lysate
    MMP-9 Antibody - BSA Free NBP1-57940
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse glioma tissue
    Species: Mouse
    Verified Customer | Posted 07/21/2014
    Western Blot Image of MMP-9
    MMP-9 Antibody - BSA Free NBP1-57940

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MMP-9 Antibody - BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q:  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 

    A:

    We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.
    It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.
    6 have been tested for detection, seen here.

  • Q: I have purchased MMP-9 NBP1-57940 and it is lyophilized. I would like to know how to dilute them for further processing.

    A: Our product NBP1-57940 is provided lyophilized. We recommend reconstituting with 0.1ml of dH2O.

  • Q:  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 

    A:

    We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.
    It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.
    6 have been tested for detection, seen here.

  • Q: I have purchased MMP-9 NBP1-57940 and it is lyophilized. I would like to know how to dilute them for further processing.

    A: Our product NBP1-57940 is provided lyophilized. We recommend reconstituting with 0.1ml of dH2O.

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