MORC2 Antibody - BSA Free
Novus Biologicals | Catalog # NB600-236
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
A synthetic peptide, which maps to the region between residues 600-700 of human zinc finger, CW-type with coiled-coil domain 1 using the numbering given in entry NP_055756.1 (GeneID 22880).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MORC2 Antibody - BSA Free
Western Blot: MORC2 Antibody [NB600-236]
Western Blot: MORC2 Antibody [NB600-236] - Detection of human MORC2 (ZCWCC1) by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MORC2 antibody [NB600-236] used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunoprecipitation: MORC2 Antibody [NB600-236]
Immunoprecipitation: MORC2 Antibody [NB600-236] - Detection of human MORC2 (ZCWCC1) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MORC2 antibody NB600-236 used for IP at 3 ug per reaction. MORC2 was also immunoprecipitated by rabbit anti-MORC2 antibody NB600-237. For blotting immunoprecipitated MORC2, NB600-236 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for MORC2 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg of lysate
Western Blot
1:2500-1:10000
Application Notes
By Western Blot, this antibody recognizes a band at ~111 kDa, representing ZCWCC1 protein. May be used for immunoprecipitation, as well. Suggested working dilutions: * Western Blot - 1:2,500-1:10,000 Immunoprecipitation - 2-5 ug/mg lysate Immunohistochemistry - ND *The investigator should determine the optimal working dilution for a specific application.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MORC2
Alternate Names
AC004542.C22.1, KIAA0852, MORC family CW-type zinc finger 2, MORC family CW-type zinc finger protein 2, ZCW3, ZCWCC1, Zinc finger CW-type coiled-coil domain protein 1, zinc finger, CW type with coiled-coil domain 1, zinc finger, CW-type with coiled-coil domain 1
Entrez Gene IDs
22880 (Human)
Gene Symbol
MORC2
UniProt
Additional MORC2 Products
Product Documents for MORC2 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for MORC2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for MORC2 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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