Mouse Autoimmune Regulator/AIRE Alexa Fluor® 488-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse AIRE in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human AIRE is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 609930
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse AIRE
    Ser476-Ser552 (predicted)
    Accession # Q9Z0E3
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Autoimmune Regulator/AIRE in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Autoimmune Regulator/AIRE Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC6184G, filled histogram) or isotype control antibody (Catalog # IC006G, open histogram). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Autoimmune Regulator/AIRE

Autoimmune Regulator (AIRE) is an approximately 60 kDa nuclear and cytosolic protein that is required for the development of T cell tolerance. It regulates the expression of self-antigens by both thymic epithelial cells and extrathymic cell types. Genes known to be regulated by AIRE include FABP2, Proinsulin, Oxytocin, MCP-2, S100A8 and Cytochrome p450. The genes, referred to as TRAs, represent non-thymic, tissue-specific molecules that could serve as autoantigens at a later date. Their expression in the thymus (and select extrthymic sites) allows for the indentification and elimination of potentially autoreactive T cell clones. While some mouse and human molecules are identical in their regulation by AIRE, possibly not all are, and this leads to subtle differences in the autoimmune syndromes seen in human and mouse that are attributable to AIRE absence or mutation. AIRE regulates gene transcription through interactions with DNA, histone H3, and the nuclear matrix. It contains one HSR domain that is involved in dimerization (aa 1-106), a nuclear localization sequence (aa 114-134), one SAND domain involved in DNA binding (aa 182-282), and two PHD zinc finger domains that mediate protein-protein interactions (aa 298-345 and aa 433-475). Alternate splicing of mouse AIRE generates at least eleven isoforms. Within aa 476-552, mouse AIRE shares 91% and 64% aa sequence identity with rat and human AIRE, respectively.

  • Entrez Gene IDs:
    326 (Human); 11634 (Mouse); 294328 (Rat)
  • Alternate Names:
    AIRE; APECED protein; APECED; APECEDAPSI; APS1; APSI; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; autoimmune regulator (APECED protein)10APS1AIRE1; autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermaldystrophy); autoimmune regulator; PGA1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Alexa Fluor® 488‑conjugated Isotype Control

Ctrl IC006G  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
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