Mouse Autoimmune Regulator/AIRE Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse AIRE in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human AIRE is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 609930
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse AIRE
    Ser476-Ser552 (predicted)
    Accession # Q9Z0E3
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Autoimmune Regulator/AIRE by Western Blot. Western blot shows lysates of mouse splenocytes and mouse thymus tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Autoimmune Regulator/AIRE Monoclonal Antibody (Catalog # MAB6184) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for Autoimmune Regulator/AIRE at approximately 70 and 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Intracellular Staining by Flow Cytometry
Detection of Autoimmune Regulator/AIRE in Mouse splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse Autoimmune Regulator/AIRE Monoclonal Antibody (Catalog # MAB6184, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).  To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

 

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Autoimmune Regulator/AIRE

AutoImmune REgulator (AIRE) is an approximately 60 kDa nuclear and cytosolic protein that is required for the development of T cell tolerance. It regulates the expression of self-antigens by thymic epithelial cells, and mutations in AIRE are causative of the autoimmune disorder, APECED. AIRE regulates gene transcription through interactions with DNA, histone H3, and the nuclear matrix. It contains one HSD domain (aa 1-105), a nuclear localization sequence (aa 113-133), one SAND domain (aa 181-280), and two PHD zinc finger domains (aa 299-340 and aa 434-475). Alternate splicing of human AIRE generates isoforms that lack the HSR and SAND domains and/or the second PHD domain. Within aa 476-552, human AIRE shares 65% and 63% aa sequence identity with mouse and rat AIRE, respectively.

  • Entrez Gene IDs:
    326 (Human); 11634 (Mouse); 294328 (Rat)
  • Alternate Names:
    AIRE; APECED protein; APECED; APECEDAPSI; APS1; APSI; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; autoimmune regulator (APECED protein)10APS1AIRE1; autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermaldystrophy); Autoimmune Regulator; PGA1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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