Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 609930
View all Autoimmune Regulator/AIRE Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse AIRE
Ser476-Ser552 (predicted)
Accession # Q9Z0E3

Specificity

Detects mouse AIRE in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human AIRE is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Autoimmune Regulator/AIRE Antibody

Detection of Mouse Autoimmune Regulator/AIRE antibody by Western Blot.

Detection of Mouse Autoimmune Regulator/AIRE by Western Blot.

Western blot shows lysates of mouse splenocytes and mouse thymus tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Autoimmune Regulator/AIRE Monoclonal Antibody (Catalog # MAB6184) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for Autoimmune Regulator/AIRE at approximately 70 and 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Autoimmune Regulator/AIRE antibody in Mouse splenocytes antibody by Flow Cytometry.

Detection of Autoimmune Regulator/AIRE in Mouse splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse Autoimmune Regulator/AIRE Monoclonal Antibody (Catalog # MAB6184, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Mouse Autoimmune Regulator/AIRE Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample:

Mouse splenocytes fixed with paraformaldehyde and permeabilized with saponin.

Western Blot

1 µg/mL
Sample: Mouse splenocytes and mouse thymus tissue

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Autoimmune Regulator/AIRE

AutoImmune REgulator (AIRE) is an approximately 60 kDa nuclear and cytosolic protein that is required for the development of T cell tolerance. It regulates the expression of self-antigens by thymic epithelial cells, and mutations in AIRE are causative of the autoimmune disorder, APECED. AIRE regulates gene transcription through interactions with DNA, histone H3, and the nuclear matrix. It contains one HSD domain (aa 1-105), a nuclear localization sequence (aa 113-133), one SAND domain (aa 181-280), and two PHD zinc finger domains (aa 299-340 and aa 434-475). Alternate splicing of human AIRE generates isoforms that lack the HSR and SAND domains and/or the second PHD domain. Within aa 476-552, human AIRE shares 65% and 63% aa sequence identity with mouse and rat AIRE, respectively.

Alternate Names

AIRE, APECED, APS1, APSI, PGA1

Entrez Gene IDs

326 (Human); 11634 (Mouse); 294328 (Rat)

Gene Symbol

AIRE

UniProt

Q9Z0E3

Additional Autoimmune Regulator/AIRE Products

Product Documents for Mouse Autoimmune Regulator/AIRE Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Autoimmune Regulator/AIRE Antibody

For research use only

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