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Mouse Axl Alexa Fluor® 700-conjugated Antibody

    
Datasheet / CoA / SDS
Detection of Axl in ST‑2 Mouse Cell Line by Flow Cytometry.

Flow Cytometry

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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Axl.
  • Source
    Monoclonal Rat IgG2A Clone # 175128
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Axl
    Ala19-Trp445
    Accession # Q00993
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
Product Datasheets
Product Datasheet

COA

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SDS
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Flow Cytometry
Flow Cytometry Axl Antibody (175128) [Alexa Fluor® 700]
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Detection of Axl in ST‑2 Mouse Cell Line by Flow Cytometry. ST‑2 mouse bone marrow-derived stromal cell line was stained with Rat Anti-Mouse Axl Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB8541N, filled histogram) or isotype control antibody (Catalog # IC006N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Axl

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The mouse Axl cDNA encodes an 888 amino acid (aa) precursor that includes an 18 aa signal sequence, a 427 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of mouse and human Axl share 81% aa sequence identity. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

  • References:
    1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
    2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
    3. Holland, S. et al. (2005) Canc. Res. 65:9294.
  • Long Name:
    Axl Receptor Tyrosine Kinase
  • Entrez Gene IDs:
    558 (Human); 26362 (Mouse)
  • Alternate Names:
    Ark; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; Axl; EC 2.7.10; EC 2.7.10.1; JTK11; tyrosine-protein kinase receptor UFO; Ufo; UFOoncogene AXL
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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Alexa Fluor® 700-conjugated Antibody

 Ctrl IC006N
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

 Flow FC001 10
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Flow Cytometry Mouse Lyse Buffer (10X)

 Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

 Flow FC002 1
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