Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 175128
View all Axl Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Axl
Ala19-Trp445
Accession # Q00993

Specificity

Detects mouse Axl.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Axl APC‑conjugated Antibody

Detection of Axl antibody in ST-2 Mouse Cell Line antibody by Flow Cytometry.

Detection of Axl in ST‑2 Mouse Cell Line by Flow Cytometry.

ST-2 mouse bone marrow-derived stromal cell line was stained with Rat Anti-Mouse Axl APC-conjugated Monoclonal Antibody (Catalog # FAB8541A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Axl APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: ST‑2 mouse bone marrow-derived stromal cell line

Reviewed Applications

Read 1 review rated 4 using FAB8541A in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Axl

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The mouse Axl cDNA encodes an 888 amino acid (aa) precursor that includes an 18 aa signal sequence, a 427 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of mouse and human Axl share 81% aa sequence identity. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

References

  1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
  2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  3. Holland, S. et al. (2005) Canc. Res. 65:9294.

Long Name

Axl Receptor Tyrosine Kinase

Alternate Names

AI323647, ARK, JTK11, Tyro7, UFO

Entrez Gene IDs

558 (Human); 26362 (Mouse); 102126361 (Cynomolgus Monkey)

Gene Symbol

AXL

UniProt

Q00993

Additional Axl Products

Product Documents for Mouse Axl APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Axl APC‑conjugated Antibody

For research use only

Citations for Mouse Axl APC‑conjugated Antibody

Customer Reviews for Mouse Axl APC‑conjugated Antibody (1)

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  • Mouse Axl APC-conjugated Antibody
    Name: Adam Guess
    Application: Flow Cytometry
    Sample Tested: Bone marrow cells
    Species: Mouse
    Verified Customer | Posted 08/01/2016
    Mouse Axl APC‑conjugated Antibody FAB8541A

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