Mouse Axl Antibody

Catalog # Availability Size / Price Qty
MAB8541
MAB8541-SP
Mouse Axl Antibody in Data
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Product Details
Citations (5)
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Mouse Axl Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Axl.
Source
Monoclonal Rat IgG2A Clone # 175128
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Axl
Ala19-Trp445
Accession # Q00993
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Axl in ST‑2 Mouse Cell Line by Flow Cytometry. View Larger

Detection of Axl in ST‑2 Mouse Cell Line by Flow Cytometry. ST-2 mouse bone marrow-derived stromal cell line was stained with Rat Anti-Mouse Axl Monoclonal Antibody (Catalog # MAB8541, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Axl

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The mouse Axl cDNA encodes an 888 amino acid (aa) precursor that includes an 18 aa signal sequence, a 427 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of mouse and human Axl share 81% aa sequence identity. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

References
  1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
  2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  3. Holland, S. et al. (2005) Canc. Res. 65:9294.
Long Name
Axl Receptor Tyrosine Kinase
Entrez Gene IDs
558 (Human); 26362 (Mouse)
Alternate Names
AI323647; ARK; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; Axl; EC 2.7.10; EC 2.7.10.1; JTK11; Tyro7; tyrosine-protein kinase receptor UFO; UFO; UFOoncogene AXL

Product Datasheets

Citations for Mouse Axl Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Pan-TAM tyrosine kinase inhibitor BMS-777607 enhances anti-PD-1 mAb efficacy in a murine model of triple-negative breast cancer
    Authors: RB Birge, C Kasikara, V Davra, D Calianese, K Geng, TE Spires, M Quigley, M Wichroski, G Sriram, L Suarez-Lop, MB Yaffe, SV Kotenko, MS De Lorenzo
    Cancer Res., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. BCL6 promotes glioma and serves as a therapeutic target
    Authors: L Xu, Y Chen, M Dutra-Clar, A Mayakonda, M Hazawa, SE Savinoff, N Doan, JW Said, WH Yong, A Watkins, H Yang, LW Ding, YY Jiang, JW Tyner, J Ching, JP Kovalik, V Madan, SL Chan, M Müschen, JJ Breunig, DC Lin, HP Koeffler
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. The E3 ligase Cbl-b and TAM receptors regulate cancer metastasis via natural killer cells.
    Authors: Paolino, Magdalen, Choidas, Axel, Wallner, Stephani, Pranjic, Blanka, Uribesalgo, Iris, Loeser, Stefanie, Jamieson, Amanda M, Langdon, Wallace, Ikeda, Fumiyo, Fededa, Juan Pab, Cronin, Shane J, Nitsch, Roberto, Schultz-Fademrecht, Carsten, Eickhoff, Jan, Menninger, Sascha, Unger, Anke, Torka, Robert, Gruber, Thomas, Hinterleitner, Reinhard, Baier, Gottfrie, Wolf, Dominik, Ullrich, Axel, Klebl, Bert M, Penninger, Josef M
    Nature, 2014;507(7493):508-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. GAS6 receptor status is associated with dormancy and bone metastatic tumor formation.
    Authors: Taichman, Russell, Patel, Lalit R, Bedenis, Rachel, Wang, Jingchen, Weidner, Savannah, Schumann, Taibrian, Yumoto, Kenji, Berry, Janice E, Shiozawa, Yusuke, Pienta, Kenneth
    PLoS ONE, 2013;8(4):e61873.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. The Axl receptor tyrosine kinase is a discriminator of macrophage function in the inflamed lung.
    Authors: Fujimori T, Grabiec A, Kaur M, Bell T, Fujino N, Cook P, Svedberg F, MacDonald A, Maciewicz R, Singh D, Hussell T
    Mucosal Immunol, 0;8(5):1021-30.

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