Mouse B220/CD45R PerCP-conjugated Antibody

FAB1217C has been discontinued. View all B220/CD45R products.
    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse B220/CD45R.
  • Source
    Monoclonal Rat IgG2A Clone # RA3-6B2
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Abelson mouse leukemia virus-induced pre-B tumor cells
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4841F) and either (A) Rat Anti-Mouse B220/CD45R PerCP‑conjugated Monoclonal Antibody (Catalog # FAB1217C) or (B) Rat IgG2A PerCP Isotype Control (Catalog # IC006C). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: B220/CD45R

B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).

  • References:
    1. Coffman, R.L. (1982) Immuno. Rev. 69:5.
    2. Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
    3. Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
    4. Allman, D.M. et al. (1992) J. Immunol. 149:2533.
    5. Asensi, V.K. et al. (1989) Immunology 68:204.
    6. Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.
  • Long Name:
    Cluster of Differentiation 45
  • Entrez Gene IDs:
    5788 (Human); 19264 (Mouse); 24699 (Rat)
  • Alternate Names:
    B220; CD45 antigen; CD45R; EC 3.1.3.48; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide
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