Mouse BAMBI/NMA Antibody

Detection of Mouse BAMBI/NMA by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse BAMBI/NMA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2387) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BAMBI/NMA at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse BAMBI/NMA by Western Blot Knockdown of BAMBI inhibited myogenic differentiation. All the cell samples were harvested after transfection and myogenic induction for 48 and 96 h. (a) The western blot images of BAMBI and GAPDH; (b) the efficiency of siRNA interference on the mRNA and protein expression of BAMBI; (c) the mRNA expression of MyoD at 48 h and that of MyoG and MyHC at 96 h; (d) the western blot images of MyoD at 48 h, MyHC at 96 h, and their corresponding GAPDH; (e) the protein expression of MyoD at 48 h and MyHC at 96 h; (f) immunofluorescence of MyHC in C2C12 myotubes at 96 h post differentiation, images captured at 100× magnification; (g) the populations of myotubes; (h) the differentiation index; and (i) the myotube fusion index. The results were represented as mean ± SD; n = 3; * p < 0.05; ** p < 0.01. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/16/8/17734), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse BAMBI/NMA by Western Blot Knockdown of BAMBI suppressed Wnt/ beta -catenin signaling. All the cell samples were harvested after transfection and myogenic induction for 48 h. (a) The western blot images of nuclear beta -catenin and laminB1; (b) the nuclear beta -catenin protein levels; (c) the mRNA expression of Axin2; (d) the western blot images of nuclear beta -catenin and laminB1; (e) the nuclear beta -catenin protein levels; (f) the mRNA expression of Axin2; (g) the western blot images of BAMBI and GAPDH; and (h) the mRNA and protein expression of BAMBI. The results were represented as mean ± SD; n = 3; * p < 0.05; ** p < 0.01. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/16/8/17734), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human BAMBI/NMA by Western Blot Rapamycin-induced autophagy in HUVEC.Western blot for BAMBI, GAPDH, and LC3 in BAMBI overexpressing HUVECs cultured in 10% FBS and treated with vehicle (CTRL), rapamycin, bafilomycin or bafilomycin plus rapamycin for 24 hours. Rapamycin alone reduces the BAMBI band, whereas bafilomycin alone and even in combination with rapamycin enhances it. Rapamycin causes a shift from the LC3-I to the LC3-II band, and bafilomycin alone enhances both LC3 bands. Bafilomycin does not, however, prevent the shift from LC3-I to LC3-II induced by rapamycin. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/20886049), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human BAMBI/NMA by Western Blot BAMBI protein levels in overexpressing HUVECs.7A. Representative Western blot and densitometry analysis for BAMBI in HUVECs overexpressing BAMBI (lentiviral system). Cells were cultured in 10% FBS or 0.2% FBS for 24 hours prior to protein extraction. GAPDH is shown as loading control. 7B. For densitometry analysis, normalized relative intensities were calculated from band intensity values obtained using the NIH Image J program. Data are means +/− SEM of relative BAMBI∶GAPDH ratios (normalized to 10% FBS control cells) for two independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/20886049), licensed under a CC-BY license. Not internally tested by R&D Systems.