BAMBI/NMA is a type I transmembrane protein that shares sequence homology with the TGF-beta type I receptor family protein but has a short cytoplasmic region that lacks the kinase domain. It functions as a membrane-bound decoy receptor by competing with type I receptors to form a heterodimer with type II receptors. Mouse BAMBI/NMA share approximately 93% amino acid sequence homology with human BAMBI/NMA.
Discontinued Product
AF2387 has been discontinued.
View all BAMBI/NMA products.
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse BAMBI/NMA
Ile28-Thr143
Accession # Q9D0L6
Ile28-Thr143
Accession # Q9D0L6
Specificity
Detects mouse BAMBI/NMA in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human BAMBI is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse BAMBI/NMA Antibody
Detection of Mouse BAMBI/NMA by Western Blot.
Western blot shows lysates of mouse liver tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse BAMBI/NMA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2387) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BAMBI/NMA at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse BAMBI/NMA by Western Blot
Knockdown of BAMBI inhibited myogenic differentiation. All the cell samples were harvested after transfection and myogenic induction for 48 and 96 h. (a) The western blot images of BAMBI and GAPDH; (b) the efficiency of siRNA interference on the mRNA and protein expression of BAMBI; (c) the mRNA expression of MyoD at 48 h and that of MyoG and MyHC at 96 h; (d) the western blot images of MyoD at 48 h, MyHC at 96 h, and their corresponding GAPDH; (e) the protein expression of MyoD at 48 h and MyHC at 96 h; (f) immunofluorescence of MyHC in C2C12 myotubes at 96 h post differentiation, images captured at 100× magnification; (g) the populations of myotubes; (h) the differentiation index; and (i) the myotube fusion index. The results were represented as mean ± SD; n = 3; * p < 0.05; ** p < 0.01. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/16/8/17734), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse BAMBI/NMA by Western Blot
Knockdown of BAMBI suppressed Wnt/ beta -catenin signaling. All the cell samples were harvested after transfection and myogenic induction for 48 h. (a) The western blot images of nuclear beta -catenin and laminB1; (b) the nuclear beta -catenin protein levels; (c) the mRNA expression of Axin2; (d) the western blot images of nuclear beta -catenin and laminB1; (e) the nuclear beta -catenin protein levels; (f) the mRNA expression of Axin2; (g) the western blot images of BAMBI and GAPDH; and (h) the mRNA and protein expression of BAMBI. The results were represented as mean ± SD; n = 3; * p < 0.05; ** p < 0.01. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/16/8/17734), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human BAMBI/NMA by Western Blot
Rapamycin-induced autophagy in HUVEC.Western blot for BAMBI, GAPDH, and LC3 in BAMBI overexpressing HUVECs cultured in 10% FBS and treated with vehicle (CTRL), rapamycin, bafilomycin or bafilomycin plus rapamycin for 24 hours. Rapamycin alone reduces the BAMBI band, whereas bafilomycin alone and even in combination with rapamycin enhances it. Rapamycin causes a shift from the LC3-I to the LC3-II band, and bafilomycin alone enhances both LC3 bands. Bafilomycin does not, however, prevent the shift from LC3-I to LC3-II induced by rapamycin. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/20886049), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human BAMBI/NMA by Western Blot
BAMBI protein levels in overexpressing HUVECs.7A. Representative Western blot and densitometry analysis for BAMBI in HUVECs overexpressing BAMBI (lentiviral system). Cells were cultured in 10% FBS or 0.2% FBS for 24 hours prior to protein extraction. GAPDH is shown as loading control. 7B. For densitometry analysis, normalized relative intensities were calculated from band intensity values obtained using the NIH Image J program. Data are means +/− SEM of relative BAMBI∶GAPDH ratios (normalized to 10% FBS control cells) for two independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/20886049), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse BAMBI/NMA Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Mouse liver tissue
Sample: Mouse liver tissue
Reviewed Applications
Read 3 reviews rated 4.3 using AF2387 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: BAMBI/NMA
Long Name
BMP and Activin Membrane-bound Inhibitor
Alternate Names
NMA
Gene Symbol
BAMBI
UniProt
Additional BAMBI/NMA Products
Product Documents for Mouse BAMBI/NMA Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse BAMBI/NMA Antibody
For research use only
Related Research Areas
Citations for Mouse BAMBI/NMA Antibody
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4.3 out of 5
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Application: Western BlotSample Tested: Lung tissueSpecies: MouseVerified Customer | Posted 07/19/2021
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Application: Western BlotSample Tested: Liver tissueSpecies: MouseVerified Customer | Posted 06/30/2021
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Application: Western BlotSample Tested: Adult lungSpecies: MouseVerified Customer | Posted 06/10/2019
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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