Mouse BAMBI/NMA Antibody

Catalog # Availability Size / Price Qty
AF2387
AF2387-SP

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Detection of Mouse BAMBI/NMA by Western Blot.
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (3)

Mouse BAMBI/NMA Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse BAMBI/NMA in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human BAMBI is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse BAMBI/NMA
Ile28-Thr143
Accession # Q9D0L6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse BAMBI/NMA antibody by Western Blot. View Larger

Detection of Mouse BAMBI/NMA by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse BAMBI/NMA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2387) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for BAMBI/NMA at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAMBI/NMA

BAMBI/NMA is a type I transmembrane protein that shares sequence homology with the TGF-beta type I receptor family protein but has a short cytoplasmic region that lacks the kinase domain. It functions as a membrane-bound decoy receptor by competing with type I receptors to form a heterodimer with type II receptors. Mouse BAMBI/NMA share approximately 93% amino acid sequence homology with human BAMBI/NMA.

Long Name
BMP and Activin Membrane-bound Inhibitor
Entrez Gene IDs
25805 (Human); 68010 (Mouse)
Alternate Names
BAMBI; BMP and activin membrane-bound inhibitor homolog (Xenopus laevis); BMP and activin membrane-bound inhibitor homolog; NMA; NMANon-metastatic gene A protein; Putative transmembrane protein NMA

Product Datasheets

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Citations for Mouse BAMBI/NMA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation.
    Authors: Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D
    PLoS ONE, 2010-09-24;5(9):e12995.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  2. BAMBI (bone morphogenetic protein and activin membrane-bound inhibitor) reveals the involvement of the transforming growth factor-beta family in pain modulation.
    Authors: Tramullas M, Lantero A, Diaz A, Morchon N, Merino D, Villar A, Buscher D, Merino R, Hurle JM, Izpisua-Belmonte JC, Hurle MA
    J. Neurosci., 2010-01-27;30(4):1502-11.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. TLR4 enhances TGF-beta signaling and hepatic fibrosis.
    Authors: Seki E, De Minicis S, Osterreicher CH, Kluwe J, Osawa Y, Brenner DA, Schwabe RF
    Nat. Med., 2007-10-21;13(11):1324-32.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody

WB,ELISA

Reviews for Mouse BAMBI/NMA Antibody

Average Rating: 4.3 (Based on 3 Reviews)

5 Star
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4 Star
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3 Star
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Mouse BAMBI/NMA Antibody
By Anonymous on 07/19/2021
Application: WB Sample Tested: Lung tissue Species: Mouse

Mouse BAMBI/NMA Antibody
By Anonymous on 06/30/2021
Application: WB Sample Tested: Liver tissue Species: Mouse

Mouse BAMBI/NMA Antibody
By Anonymous on 06/10/2019
Application: WB Sample Tested: Adult lung Species: Mouse