Mouse BTLA Alexa Fluor® 488-conjugated Antibody

Datasheet / CoA / SDS
Product Details
Supplemental Products
  • Species Reactivity
  • Specificity
    Detects mouse BTLA in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human BTLA is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse BTLA
    Glu30-Gly176 (Pro41Glu, Thr45Asn, Thr47Lys, Gln52His, Arg55Trp, Gln63Glu, Cys85Trp)
    Accession # Q32MV9
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Product Datasheets

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  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of BTLA in B220+ Mouse Splenocytes by Flow Cytometry. B220+ mouse splenocytes were stained with Goat Anti-Mouse BTLA Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3007G, filled histogram) or isotype control antibody (Catalog # IC108G, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: BTLA

B- and T-lymphocyte Attenuator (BTLA), also known as CD272, is a 70 kDa, Ig-superfamily, type I transmembrane glycoprotein that is structurally similar to the CD28 family of T cell co-stimulatory or coinhibitory molecules (1‑3). Unlike CD28 family members, however, the BTLA extracellular Ig domain is an I-type rather than a V-type domain, and BTLA does not form homodimers (4). BTLA also differs from CD28 family members through the interaction of its Ig domain with the TNF superfamily member HVEM (herpesvirus entry mediator; TNFSF14) rather than with B7 family ligands (5). BTLA is a coinhibitory molecule expressed on T cells, B cells and, depending on the mouse strain, macrophages, dendritic and NK cells (6). Expression is low in naïve T cells and increased during antigen-specific induction of anergy. In B cells, BTLA is highest when cells are mature and naïve (6). BTLA apparently limits T cell numbers, since deletion of BTLA results in overproduction of T cells, especially CD8+ memory T cells that are hyper-responsive to TCR crosslinking (7). The 306 amino acid (aa) BTLA contains a 29 aa signal sequence, a 154 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 102 aa cytoplasmic domain. There are two ITIM motifs and three Tyr phosphorylation sites in the cytoplasmic tail that mediate inhibitory signaling (8, 9). The binding of the BTLA to HVEM does not preclude additional binding of a mammalian stimulatory HVEM ligand, either LIGHT or lymphotoxin-alpha to the complex (4). At least three alleles varying by up to ten extracellular amino acids occur in different mouse strains (6). The ECD of C57BL/6 BTLA shows 51%, 77% and 40% aa identity to that of human, rat and canine BTLA, respectively. A splice variant lacking the Ig domain, termed BTLAs, has been reported (3).

  • References:
    1. Murphy, K. M. et al. (2006) Nat. Rev. Immunol. 6:671.
    2. Croft, M. (2005) Trends Immunol. 26:292.
    3. Watanabe, N. et al. (2003) Nat. Immunol. 4:670.
    4. Compaan, D. M. et al. (2005) J. Biol. Chem. 280:39553.
    5. Sedy, J. R. et al. (2005) Nat. Immunol. 6:90.
    6. Hurchla, M. A. et al. (2005) J. Immunol. 174:3377.
    7. Krieg, C. et al. (2007) Nat. Immunol. 8:162.
    8. Gavrieli, M. et al. (2003) Biochem. Biophys. Res. Commun. 312:1236.
    9. Chemnitz, J. M. et al. (2006) J. Immunol. 176:6603.
  • Long Name:
    B- And T-Lymphocyte Attenuator
  • Entrez Gene IDs:
    151888 (Human); 208154 (Mouse)
  • Alternate Names:
    B and T lymphocyte associated; B and T lymphocyte attenuator; B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; BTLA; BTLA1; CD272 antigen; CD272; FLJ16065; MGC129743
Related Research Areas


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Isotype Controls
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Normal Goat IgG Alexa Fluor® 488-conjugated Control

Ctrl IC108G
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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