Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 815606
View all CCDC134 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CCDC134
Ala23-Leu229
Accession # Q8C7V8

Specificity

Detects mouse CCDC134 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human CCDC134 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse CCDC134 Antibody

Detection of Mouse CCDC134 antibody by Western Blot.

Detection of Mouse CCDC134 by Western Blot.

Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CCDC134 Monoclonal Antibody (Catalog # MAB7786) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CCDC134 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse CCDC134 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: J774A.1 mouse reticulum cell sarcoma macrophage cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCDC134

Coiled-coil domain containing 134 (CCDC134) is a widely expressed glycoprotein that is secreted as a 38 kDa molecule. CCDC134 overexpression inhibits kinase activity in the Raf-1/MEK/ERK and JNK/SAPK pathways. In humans, CCDC134 polymorphisms are associated with reduced levels of Amyloid beta peptide (1-42) in the cerebrospinal fluid, a potential biomarker for Alzheimer’s disease. Alternative splicing of mouse CCDC134 generates a short isoform that lacks the N-terminal 37 amino acids including the signal peptide. Mouse CCDC134 shares 90% and 95% aa sequence identity with human and rat CCDC134, respectively.

Long Name

Coiled-coil Domain Containing 134

Alternate Names

coiled-coil domain containing 134, coiled-coil domain-containing protein 134, dJ821D11.3, FLJ22349, MGC21013

Entrez Gene IDs

79879 (Human); 76457 (Mouse); 500909 (Rat)

Gene Symbol

CCDC134

UniProt

Q8C7V8

Additional CCDC134 Products

Product Documents for Mouse CCDC134 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCDC134 Antibody

For research use only

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