CCL2/JE/MCP-1, is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 promotes Th2 polarization in CD4+ T cells, and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, downregulates activity but not receptor binding.
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Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit
R&D Systems | Catalog # MJE00B
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Lysates (50 µL), Tissue Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
0.666 pg/mL
Assay Range
7.81-500 pg/mL (Cell Culture Supernates, Tissue Lysates, Tissue Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit
The Quantikine® Mouse CCL2/JE/MCP-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse MCP-1 in cell culture supernates, tissue culture supernates, tissue lysates, serum, and plasma. It contains E. coli-expressed recombinant mouse MCP-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse MCP-1. Results obtained using natural mouse MCP-1 showed dose response curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse MCP-1.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse
Specificity
Natural and recombinant mouse MCP-1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Culture Supernates, Tissue Lysates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 36.7 | 51.8 | 149 | 37.8 | 60.0 | 155 |
| Standard Deviation | 1.45 | 1.40 | 3.66 | 2.85 | 4.37 | 7.86 |
| CV% | 4.0 | 2.7 | 2.5 | 7.5 | 7.3 | 5.1 |
Recovery for Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit
The recovery of mouse MCP-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 105 | 95-112 |
| Cell Lysis Buffer (n=4) | 88 | 79-101 |
| EDTA Plasma (n=4) | 84 | 75-93 |
| Heparin Plasma (n=4) | 93 | 80-101 |
| Serum (n=4) | 91 | 83-100 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse MCP-1 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit
Mouse CCL2/JE/MCP-1 ELISA Standard Curve
Mouse/Rat CCL2/JE/MCP-1 Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL2/JE/MCP-1
Alternate Names
GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF
Gene Symbol
CCL2
Additional CCL2/JE/MCP-1 Products
Product Documents for Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit
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Customer Reviews for Mouse CCL2/JE/MCP-1 Quantikine ELISA Kit (6)
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6 Customer Ratings
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Sample Tested: lysate from mouse spinal cordVerified Customer | Posted 09/29/2024Please check figure S10 of the following paper, Scientific Reports volume 9, Article number: 17728 (2019)
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Sample Tested: Aorta tissueVerified Customer | Posted 07/25/2023
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Sample Tested: SerumVerified Customer | Posted 05/04/2023
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Sample Tested: Serum-free Cell Culture MediaVerified Customer | Posted 12/02/2021
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Sample Tested: Serum-free Cell Culture MediaVerified Customer | Posted 10/21/2021
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Sample Tested: Cell Culture MediaVerified Customer | Posted 08/06/2018I had to dilute the samples more than recommended, but the result was as expected
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