Mouse CCL7/MCP-3/MARC Alexa Fluor® 532-conjugated Antibody

Catalog #: AF-456-NAX Datasheet / COA / SDS

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AF-456-NAX-100UG

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Mouse CCL7/MCP-3/MARC Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCL7/MCP-3/MARC in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human Eotaxin and recombinant mouse (rm) JE is observed and less than 5% cross-reactivity with rmMCP-5 is observed

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse CCL7/MCP-3/MARC
Gln24-Pro97
Accession # Q03366

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Neutralization

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CCL7/MCP-3/MARC

Mouse MARC, a member of the beta subfamily of chemokines, was initially identified as a transcript that is induced in a mouse mast cell line after Fc epsilon RI triggering by IgE plus antigen. Sequence comparisons suggest that MARC may be the mouse homologue of the human MCP-3 gene. Mouse MARC/MCP-3 expression has also been detected during murine experimental allergic encephalomyelitis in the spinal cord, and in LPS-stimulated murine WEHI -3 cells and Swiss 3T3 cells where MARC expression is glucocorticoid-attenuated. Except for one amino acid subsititution, mouse MARC is identical to mouse FIC, the product of a growth factor‑activated gene. The mouse MARC cDNA encodes a 97 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 74 amino acid residue mature protein. Mouse CCR2, a mouse chemokine receptor, has been shown to bind JE/MCP-1 with high affinity and MARC/MCP-3 with lower affinity. The E. coli-expressed mouse MARC/MCP-3 produced at R&D Systems has been shown to be a monocyte and T-lymphocyte chemoattractant.

Entrez Gene IDs

6354 (Human); 20306 (Mouse)

Alternate Names

C-C motif chemokine 7; CCL7; chemokine (C-C motif) ligand 7; FIC; MARC; MCP-3; MCP3Monocyte chemotactic protein 3; MCP-3Small-inducible cytokine A7; MGC138465; Monocyte chemoattractant protein 3; NC28SCYA6; SCYA7MGC138463; small inducible cytokine A7 (monocyte chemotactic protein 3)