Mouse CCL7/MCP-3/MARC Antibody AF-456-NA: R&D Systems

Mouse CCL7/MCP-3/MARC Antibody

(4 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCL7/MCP-3/MARC in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human Eotaxin and recombinant mouse (rm) JE is observed and less than 5% cross-reactivity with rmMCP-5 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse CCL7/MCP-3/MARC
    Gln24-Pro97
    Accession # Q03366
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse CCL7/MCP‑3/MARC (Catalog # 456-MC)
  • Neutralization
    Measured by its ability to neutralize CCL7/MCP‑3/MARC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 7.5-45 µg/mL in the presence of 2 µg/mL Recombinant Mouse CCL7/MCP‑3/MARC.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL7/MARC and Neutralization by Mouse CCL7/MARC Antibody.

Recombinant Mouse CCL7/ MARC (Catalog # 456-MC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL7/
MARC (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL7/MARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-456-NA). The ND50 is typically 7.5-45 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL7/MCP-3/MARC

Mouse MARC, a member of the beta  subfamily of chemokines, was initially identified as a transcript that is induced in a mouse mast cell line after Fc epsilon RI triggering by IgE plus antigen. Sequence comparisons suggest that MARC may be the mouse homologue of the human MCP-3 gene. Mouse MARC/MCP-3 expression has also been detected during murine experimental allergic encephalomyelitis in the spinal cord, and in LPS-stimulated murine WEHI -3 cells and Swiss 3T3 cells where MARC expression is glucocorticoid-attenuated. Except for one amino acid subsititution, mouse MARC is identical to mouse FIC, the product of a growth factor‑activated gene. The mouse MARC cDNA encodes a 97 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 74 amino acid residue mature protein. Mouse CCR2, a mouse chemokine receptor, has been shown to bind JE/MCP-1 with high affinity and MARC/MCP-3 with lower affinity. The E. coli-expressed mouse MARC/MCP-3 produced at R&D Systems has been shown to be a monocyte and T-lymphocyte chemoattractant.

  • References:
    1. Kulmburg, P.A. et al. (1992) J. Exp. Med. 176:1773.
    2. Thirion, S. et al. (1994) Biochem. Biophys. Res. Commun. 201:493.
    3. Smith, J.B. and H.R. Herschman (1995) J. Biol. Chem. 270:16756.
    4. Kurihara, T. and R. Bravo (1996) J. Biol. Chem. 271:11603.
  • Entrez Gene IDs:
    6354 (Human); 20306 (Mouse)
  • Alternate Names:
    C-C motif chemokine 7; CCL7; chemokine (C-C motif) ligand 7; FIC; MARC; MCP-3; MCP3Monocyte chemotactic protein 3; MCP-3Small-inducible cytokine A7; MGC138465; Monocyte chemoattractant protein 3; NC28SCYA6; SCYA7MGC138463; small inducible cytokine A7 (monocyte chemotactic protein 3)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. CCL7 and IRF-7 Mediate Hallmark Inflammatory and IFN Responses following Rhinovirus 1B Infection.
    Authors: Girkin J, Hatchwell L, Foster P, Johnston S, Bartlett N, Collison A, Mattes J
    J Immunol, 2015;194(10):4924-30.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  2. The expression of Fn14 via mechanical stress-activated JNK contributes to apoptosis induction in osteoblasts.
    Authors: Matsui H, Fukuno N, Kanda Y, Kantoh Y, Chida T, Nagaura Y, Suzuki O, Nishitoh H, Takeda K, Ichijo H, Sawada Y, Sasaki K, Kobayashi T, Tamura S
    , 2014;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. The CCL7-CCL2-CCR2 axis regulates IL-4 production in lungs and fungal immunity.
    Authors: Szymczak WA, Deepe GS
    J. Immunol., 2009;183(3):1964-74.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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