Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Western Blot, Neutralization, Flow Cytometry, Bioassay, In vivo assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CCL7/MCP-3/MARC Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL7/MCP-3/MARC
Gln24-Pro97
Accession # Q03366

Specificity

Detects mouse CCL7/MCP-3/MARC in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human Eotaxin and recombinant mouse (rm) JE is observed and less than 5% cross-reactivity with rmMCP-5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CCL7/MCP‑3/MARC Antibody

Chemotaxis Induced by CCL7/MARC and Neutralization by Mouse CCL7/MARC Antibody.

Chemotaxis Induced by CCL7/MARC and Neutralization by Mouse CCL7/MARC Antibody.

Recombinant Mouse CCL7/ MARC (Catalog # 456-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL7/ MARC (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL7/MARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-456-NA). The ND50 is typically 7.5-45 µg/mL.
Detection of CCL7/MCP-3/MARC by Western Blot

Detection of CCL7/MCP-3/MARC by Western Blot

CCL7‐activated JAK2/STAT1 signal in macrophages. (A) Protein abundance of JAK2 and STAT1 in abdominal aortas of saline (n = 4) and Ang II‐infused (n = 4) mice. (B) Protein abundance of JAK2, p‐JAK2, STAT1, p‐STAT1 and p‐JAK2/JAK2, p‐STAT1/STAT1 ratio in BMDMs incubated with rmCCL7 (100 ng/ml). (C) Protein abundance of STAT1, p‐STAT1 and p‐STAT1/STAT1 ratio in BMDM incubated with rmCCL7(100 ng/ml) and pre‐treated 1 hour with JAK2 inhibitor Fedratinib (1 μM). Values were represented as mean ± SEM; Student's t test was used for data analysis in (A, B). One Way ANOVA was used for data analysis in(C). *P < .05; **P < .01; ***P < .001, respectively Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34189838), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CCL7/MCP-3/MARC by Western Blot

Detection of CCL7/MCP-3/MARC by Western Blot

CCL7‐CCR1 interaction regulated macrophage phenotype through JAK2 /STAT1 pathway. (A) Protein abundance of JAK2, p‐JAK2, STAT1, p‐STAT1 and p‐JAK2/JAK2, p‐STAT1/STAT1 ratio in BMDMs incubated with rmCCL7 (100 ng/ml) and pre‐treated 1 hour with CCR1 antagonist‐BX471(1 μM and 10 μM). (B) M1 markers (iNOS, IL‐6, IL‐12A, IL‐12B, TNF‐ alpha ) mRNA level in BMDMs incubated with rmCCL7 alone or in combination with JAK2 inhibitor‐Fedratinib (1 μM) and STAT1 inhibitor‐Fludarabine (1 μM). (C) Pathological mechanism diagram of CCL7 in Ang II‐induced AAA. Values were represented as mean ± SEM; One Way ANOVA was used for data analysis in (A‐B) *P < .05; **P < .01; ***P < .001, respectively Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34189838), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CCL7/MCP-3/MARC by Western Blot

Detection of CCL7/MCP-3/MARC by Western Blot

CCL7 expression and macrophages infiltration increased in Ang II‐infused abdominal aortas. (A) Representative abdominal aorta images with ultrasound examination, the maximum abdominal aorta diameter (luminal diameter) and the maximum abdominal aorta area (luminal area). A grid on the scale represented 0.5 mm. (B) qRT‐PCR for CCL7 mRNA expression in saline (n = 6) and Ang II (n = 7) infused mice. (C) Protein levels of CCL7 in saline (n = 6) and Ang II (n = 7) infused abdominal aortas. (D) Plasma concentrations of CCL7 in saline (n = 6) and Ang II (n = 9) infused mice. (E) Representative images of macrophage infiltration in aneurysmal lesions as revealed by CD68 immunostaining. Black arrows indicated CD68‐positive location, scale bars represented 200 μm (left) and 50 μm (right images). Values were represented as mean ± SEM; Student's t test was used for data analysis in (A‐E). *P < .05; **P < .01; ***P < .001, respectively Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34189838), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CCL7/MCP‑3/MARC Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CCL7/MCP‑3/MARC (Catalog # 456-MC)

Neutralization

Measured by its ability to neutralize CCL7/MCP‑3/MARC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 7.5-45 µg/mL in the presence of 2 µg/mL Recombinant Mouse CCL7/MCP‑3/MARC.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL7/MCP-3/MARC

Mouse MARC, a member of the beta  subfamily of chemokines, was initially identified as a transcript that is induced in a mouse mast cell line after Fc epsilon RI triggering by IgE plus antigen. Sequence comparisons suggest that MARC may be the mouse homologue of the human MCP-3 gene. Mouse MARC/MCP-3 expression has also been detected during murine experimental allergic encephalomyelitis in the spinal cord, and in LPS-stimulated murine WEHI -3 cells and Swiss 3T3 cells where MARC expression is glucocorticoid-attenuated. Except for one amino acid subsititution, mouse MARC is identical to mouse FIC, the product of a growth factor‑activated gene. The mouse MARC cDNA encodes a 97 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 74 amino acid residue mature protein. Mouse CCR2, a mouse chemokine receptor, has been shown to bind JE/MCP-1 with high affinity and MARC/MCP-3 with lower affinity. The E. coli-expressed mouse MARC/MCP-3 produced at R&D Systems has been shown to be a monocyte and T-lymphocyte chemoattractant.

References

  1. Kulmburg, P.A. et al. (1992) J. Exp. Med. 176:1773.
  2. Thirion, S. et al. (1994) Biochem. Biophys. Res. Commun. 201:493.
  3. Smith, J.B. and H.R. Herschman (1995) J. Biol. Chem. 270:16756.
  4. Kurihara, T. and R. Bravo (1996) J. Biol. Chem. 271:11603.

Alternate Names

MARC, MCP-3

Entrez Gene IDs

6354 (Human); 20306 (Mouse)

Gene Symbol

CCL7

UniProt

Q03366

Additional CCL7/MCP-3/MARC Products

Product Documents for Mouse CCL7/MCP‑3/MARC Antibody

Certificate of Analysis

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Product Specific Notices for Mouse CCL7/MCP‑3/MARC Antibody

For research use only

Citations for Mouse CCL7/MCP‑3/MARC Antibody

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