Mouse CCR6 Alexa Fluor® 700-conjugated Antibody

    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCR6-transfected rat RBL cells but not the parent cell line or cells transfected with mouse CCR5.
  • Source
    Monoclonal Rat IgG2A Clone # 140706
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    RBL-1 rat basophilic leukemia cell line transfected with mouse CCR6
    Met1-Met367
    Accession # O54689
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of CCR6 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with (A) Rat Anti-Mouse CCR6 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB590N) or (B) RatIgG2A isotype control antibody (Catalog # IC006N) and Rat Anti-Mouse B220/CD45R PE‑conjugated Monoclonal Antibody (Catalog # FAB1217P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR6

CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.

  • Entrez Gene IDs:
    1235 (Human); 12458 (Mouse); 308163 (Rat)
  • Alternate Names:
    BN-1; CC-CKR-6; CCR6; CCR-6; CD196 antigen; CD196; chemokine (C-C motif) receptor 6; CKR-L3G-protein coupled receptor 29; CKRL3LARC receptor; CMKBR6STRL22; DRY6; GPR29; GPR29C-C CKR-6; GPR-CY4; GPRCY4DRY-6; lymphocyte, 22; STRL22
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Alexa Fluor® 700-conjugated Antibody

Ctrl IC006N
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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