Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Neutralization, Flow Cytometry, ELISA Development (Capture)

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 140706
View all CCR6 Primary Antibodies »

Product Specifications

Immunogen

RBL-1 rat basophilic leukemia cell line transfected with mouse CCR6
Met1-Met367
Accession # O54689

Specificity

Detects mouse CCR6-transfected rat RBL cells but not the parent cell line or cells transfected with mouse CCR5.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse CCR6 Antibody

Detection of CCR6 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CCR6 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with (A) Rat Anti-Mouse CCR6 Monoclonal Antibody (Catalog # MAB590) or (B) Rat IgG2A control antibody (Catalog # MAB006) followed by Goat anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113) and Rat Anti-Mouse B220/CD45R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1217F). View our protocol for Staining Membrane-associated Proteins.
CCR6 antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr).

CCR6 in Mouse Thymus.

CCR6 was detected in perfusion fixed frozen sections of mouse thymus using Mouse CCR6 Monoclonal Antibody (Catalog # MAB590) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
CCR6 antibody in Mouse Spleen by Immunohistochemistry (IHC-Fr).

CCR6 in Mouse Spleen.

CCR6 was detected in immersion fixed frozen sections of mouse spleen using Rat Anti-Mouse CCR6 Monoclonal Antibody (Catalog # MAB590) at 0.3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes in lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse CCR6 by Flow Cytometry

Detection of Mouse CCR6 by Flow Cytometry

Priming of pik3cg−/− CD4+ T cells is reduced during EAE.(A) Frequencies of CD4+ cells in draining LN that are CD69+ on day 9 post-immunisation for EAE as determined by flow cytometry. A representative histogram overlay gating on CD4+ cells is shown (filled = isotype control on WT, solid line = anti-CD69 on WT, dotted line = anti-CD69 on pik3cg−/−) (B) In vivo proliferation of CD4+ cells, measured by BrdU incorporation, is reduced in pik3cg−/− mice at day 9 post-immunization for EAE. (n = 6 mice per group). Representative dot plots gating on CD4+ cells are shown. (C) Expression of chemokine receptors by CD4+ T cells indicative of T cell activation was determined by flow cytometry. Representative histogram overlays showing expression of CCR7, CCR6 and CXCR3 are shown (filled = isotype control, solid line = WT, dotted line = pik3cg−/−). (D) Expression of CD62L, CD49d and PSGL-1 by CD4+ cells from spleen of day 9 immunised mice (n = 3 mice per group). All data shown are mean ± s.e.m. (*, p<0.05). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0045095), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CCR6 by Flow Cytometry

Detection of CCR6 by Flow Cytometry

Endogenous T cells accumulate in K14E7 skin with an enrichment for CCR6-expressing CD4 T cells.(A) Ear skin tissue was taken from K14E7 mice in addition to control mice expressing the SIY epitope under the K14 promoter. CD4 and CD8 expression was measured on gated CD45+CD3+ cells. Data is representative of at least 4 mice/group (B) The numbers of CD4+ and CD8+ T cells in K14E7 skin or control C57 skin were enumerated per square centimetre of ear skin using flow count beads in flow cytometry. Data represents pooled mice from at least two independent experiments. (C) Gated CD3+ CD4+ T cells from the lymph node or skin of K14E7 mice were analysed for expression of the chemokine receptors, CCR6 and CCR4. The left hand panels show representative plots of isotype and CCR6 antibody staining for CD4+ T cells while the graph summarises chemokine receptor staining representative of 6 mice/group from 3 independent experiments. (D) Both K14E7 mice and C57 mice were analysed for the proliferative marker, Ki67, using intracellular staining of lymphocytes derived from the skin and inguinal lymph nodes (iLN). Naive C57 spleen cells (negative control) or C57 spleen cells treated for 3 days with PMA/Ionomycin (positive control) were also analysed. The K14E7 and C57 data represent 7 mice/group derived from 3 independent experiments while controls are spleen cells from a single mouse in 3 independent experiments. The lower right hand panels are representative plots showing isotype and Ki67 staining in K14E7 or C57 mouse skin. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23469070), licensed under a CC0-1.0 license. Not internally tested by R&D Systems.
Detection of CCR6 by Flow Cytometry

Detection of CCR6 by Flow Cytometry

CCR6 expression is highest in TFR cells amongst follicular T cell populations. (A, B) Representative gating strategy for CCR6+ TFH cells (CD4+B220-CXCR5hiPD-1hiFoxp3-), TFR cells (CD4+B220-CXCR5hiPD-1hiFoxp3+), naïve CD4 T cells (CD4+B220-CD44loFoxp3-) and natural T-regulatory cells (nTreg: CD4+B220-CD44midFoxp3+Nrp-1+) 6 days after SRBC immunization. (C) Geometrical mean fluorescence intensity (gMFI) of CCR6 and, (D) percentage of CCR6+ cells within populations from (A) and (B). (E) Representative gating strategy for TFH (Foxp3-) and TFR cells (Foxp3+) within CCR6+ follicular T cells (CD4+B220-CXCR5hiPD-1hiCCR6+). (F) Ratio of TFH : TFR cells within gating strategies from (A, E). (G) Frequency of CCR6+ TFH and TFR cells at indicated time points after i.p. NP-KLH/Alum immunization. (A–C, E, F) Data representative of two independent experiments, n=4 mice ± SEM, one-way ANOVA with Tukey’s multiple comparisons test, (G) n=4-5 mice per time-point ± SEM, two-way ANOVA with Sidak’s multiple comparison test. NS, Not significant, *p < 0.05, ***p < 0.001, ****p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35812408), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CCR6 by Flow Cytometry

Detection of CCR6 by Flow Cytometry

CCR6 expression is highest in TFR cells amongst follicular T cell populations. (A, B) Representative gating strategy for CCR6+ TFH cells (CD4+B220-CXCR5hiPD-1hiFoxp3-), TFR cells (CD4+B220-CXCR5hiPD-1hiFoxp3+), naïve CD4 T cells (CD4+B220-CD44loFoxp3-) and natural T-regulatory cells (nTreg: CD4+B220-CD44midFoxp3+Nrp-1+) 6 days after SRBC immunization. (C) Geometrical mean fluorescence intensity (gMFI) of CCR6 and, (D) percentage of CCR6+ cells within populations from (A) and (B). (E) Representative gating strategy for TFH (Foxp3-) and TFR cells (Foxp3+) within CCR6+ follicular T cells (CD4+B220-CXCR5hiPD-1hiCCR6+). (F) Ratio of TFH : TFR cells within gating strategies from (A, E). (G) Frequency of CCR6+ TFH and TFR cells at indicated time points after i.p. NP-KLH/Alum immunization. (A–C, E, F) Data representative of two independent experiments, n=4 mice ± SEM, one-way ANOVA with Tukey’s multiple comparisons test, (G) n=4-5 mice per time-point ± SEM, two-way ANOVA with Sidak’s multiple comparison test. NS, Not significant, *p < 0.05, ***p < 0.001, ****p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35812408), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CCR6 by Flow Cytometry

Detection of CCR6 by Flow Cytometry

CCR6 expression is highest in TFR cells amongst follicular T cell populations. (A, B) Representative gating strategy for CCR6+ TFH cells (CD4+B220-CXCR5hiPD-1hiFoxp3-), TFR cells (CD4+B220-CXCR5hiPD-1hiFoxp3+), naïve CD4 T cells (CD4+B220-CD44loFoxp3-) and natural T-regulatory cells (nTreg: CD4+B220-CD44midFoxp3+Nrp-1+) 6 days after SRBC immunization. (C) Geometrical mean fluorescence intensity (gMFI) of CCR6 and, (D) percentage of CCR6+ cells within populations from (A) and (B). (E) Representative gating strategy for TFH (Foxp3-) and TFR cells (Foxp3+) within CCR6+ follicular T cells (CD4+B220-CXCR5hiPD-1hiCCR6+). (F) Ratio of TFH : TFR cells within gating strategies from (A, E). (G) Frequency of CCR6+ TFH and TFR cells at indicated time points after i.p. NP-KLH/Alum immunization. (A–C, E, F) Data representative of two independent experiments, n=4 mice ± SEM, one-way ANOVA with Tukey’s multiple comparisons test, (G) n=4-5 mice per time-point ± SEM, two-way ANOVA with Sidak’s multiple comparison test. NS, Not significant, *p < 0.05, ***p < 0.001, ****p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35812408), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CCR6 by Flow Cytometry

Detection of CCR6 by Flow Cytometry

CCR6 expression is highest in TFR cells amongst follicular T cell populations. (A, B) Representative gating strategy for CCR6+ TFH cells (CD4+B220-CXCR5hiPD-1hiFoxp3-), TFR cells (CD4+B220-CXCR5hiPD-1hiFoxp3+), naïve CD4 T cells (CD4+B220-CD44loFoxp3-) and natural T-regulatory cells (nTreg: CD4+B220-CD44midFoxp3+Nrp-1+) 6 days after SRBC immunization. (C) Geometrical mean fluorescence intensity (gMFI) of CCR6 and, (D) percentage of CCR6+ cells within populations from (A) and (B). (E) Representative gating strategy for TFH (Foxp3-) and TFR cells (Foxp3+) within CCR6+ follicular T cells (CD4+B220-CXCR5hiPD-1hiCCR6+). (F) Ratio of TFH : TFR cells within gating strategies from (A, E). (G) Frequency of CCR6+ TFH and TFR cells at indicated time points after i.p. NP-KLH/Alum immunization. (A–C, E, F) Data representative of two independent experiments, n=4 mice ± SEM, one-way ANOVA with Tukey’s multiple comparisons test, (G) n=4-5 mice per time-point ± SEM, two-way ANOVA with Sidak’s multiple comparison test. NS, Not significant, *p < 0.05, ***p < 0.001, ****p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35812408), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CCR6 by Flow Cytometry

Detection of CCR6 by Flow Cytometry

CCR6 expression is highest in TFR cells amongst follicular T cell populations. (A, B) Representative gating strategy for CCR6+ TFH cells (CD4+B220-CXCR5hiPD-1hiFoxp3-), TFR cells (CD4+B220-CXCR5hiPD-1hiFoxp3+), naïve CD4 T cells (CD4+B220-CD44loFoxp3-) and natural T-regulatory cells (nTreg: CD4+B220-CD44midFoxp3+Nrp-1+) 6 days after SRBC immunization. (C) Geometrical mean fluorescence intensity (gMFI) of CCR6 and, (D) percentage of CCR6+ cells within populations from (A) and (B). (E) Representative gating strategy for TFH (Foxp3-) and TFR cells (Foxp3+) within CCR6+ follicular T cells (CD4+B220-CXCR5hiPD-1hiCCR6+). (F) Ratio of TFH : TFR cells within gating strategies from (A, E). (G) Frequency of CCR6+ TFH and TFR cells at indicated time points after i.p. NP-KLH/Alum immunization. (A–C, E, F) Data representative of two independent experiments, n=4 mice ± SEM, one-way ANOVA with Tukey’s multiple comparisons test, (G) n=4-5 mice per time-point ± SEM, two-way ANOVA with Sidak’s multiple comparison test. NS, Not significant, *p < 0.05, ***p < 0.001, ****p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35812408), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CCR6 by Flow Cytometry

Detection of CCR6 by Flow Cytometry

CCR6 expression is highest in TFR cells amongst follicular T cell populations. (A, B) Representative gating strategy for CCR6+ TFH cells (CD4+B220-CXCR5hiPD-1hiFoxp3-), TFR cells (CD4+B220-CXCR5hiPD-1hiFoxp3+), naïve CD4 T cells (CD4+B220-CD44loFoxp3-) and natural T-regulatory cells (nTreg: CD4+B220-CD44midFoxp3+Nrp-1+) 6 days after SRBC immunization. (C) Geometrical mean fluorescence intensity (gMFI) of CCR6 and, (D) percentage of CCR6+ cells within populations from (A) and (B). (E) Representative gating strategy for TFH (Foxp3-) and TFR cells (Foxp3+) within CCR6+ follicular T cells (CD4+B220-CXCR5hiPD-1hiCCR6+). (F) Ratio of TFH : TFR cells within gating strategies from (A, E). (G) Frequency of CCR6+ TFH and TFR cells at indicated time points after i.p. NP-KLH/Alum immunization. (A–C, E, F) Data representative of two independent experiments, n=4 mice ± SEM, one-way ANOVA with Tukey’s multiple comparisons test, (G) n=4-5 mice per time-point ± SEM, two-way ANOVA with Sidak’s multiple comparison test. NS, Not significant, *p < 0.05, ***p < 0.001, ****p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35812408), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CCR6 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse splenocytes

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse thymus, and immersion fixed frozen sections of mouse spleen

Reviewed Applications

Read 1 review rated 5 using MAB590 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCR6

CCR6 is a seven transmembrane-spanning G protein-coupled receptor that binds the chemokine MIP-3 alpha and mediates the chemoattraction of B and T lymphocytes.

Alternate Names

CC-CKR-6, CCR6, CD196, CKR-L3, CKRL3, CMKBR6, DRY6, GPR-CY4, GPR29, GPRCY4, STRL22

Entrez Gene IDs

1235 (Human); 12458 (Mouse); 308163 (Rat)

Gene Symbol

CCR6

UniProt

O54689

Additional CCR6 Products

Product Documents for Mouse CCR6 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse CCR6 Antibody

For research use only

Citations for Mouse CCR6 Antibody

Customer Reviews for Mouse CCR6 Antibody (1)

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  • Mouse CCR6 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: T cells
    Species: Mouse
    Verified Customer | Posted 08/04/2016
    murine splenic CD4 T cells were stained.
    Mouse CCR6 Antibody MAB590

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